High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production

Blum, Ailisa, Benfield, Aurelie H., Stiller, Jiri, Kazan, Kemal, Batley, Jacqueline and Gardiner, Donald M. (2016) High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production. Fungal Genetics and Biology, 90 1-11. doi:10.1016/j.fgb.2016.02.005


Author Blum, Ailisa
Benfield, Aurelie H.
Stiller, Jiri
Kazan, Kemal
Batley, Jacqueline
Gardiner, Donald M.
Title High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production
Formatted title
High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production
Journal name Fungal Genetics and Biology   Check publisher's open access policy
ISSN 1096-0937
1087-1845
Publication date 2016-05-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.fgb.2016.02.005
Open Access Status Not Open Access
Volume 90
Start page 1
End page 11
Total pages 11
Place of publication Maryland Heights, MO United States
Publisher Academic Press
Collection year 2017
Language eng
Formatted abstract
Fusarium head blight and crown rot, caused by the fungal plant pathogen Fusarium graminearum, impose a major threat to global wheat production. During the infection, plants are contaminated with mycotoxins such as deoxynivalenol (DON), which can be toxic for humans and animals. In addition, DON is a major virulence factor during wheat infection. However, it is not fully understood how DON production is regulated in F. graminearum. In order to identify regulators of DON production, a high-throughput mutant screen using Fluorescence Activated Cell Sorting (FACS) of a mutagenised TRI5-GFP reporter strain was established and a mutant over-producing DON under repressive conditions identified. A gain-of-function mutation in the F. graminearum adenylyl cyclase (FAC1), which is a known positive regulator of DON production, was identified as the cause of this phenotype through genome sequencing and segregation analysis. Our results show that the high-throughput mutant screening procedure developed here can be applied for identification of fungal proteins involved in diverse processes.
Keyword Gibberella zeae
DON
Trichothecene
Fluorescence activated cell sorting
Flow cytometry
cAMP
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
QAAFI Biological Information Technology (QBIT) Publications
 
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