Adrenocortical function in cane toads from different environments

Hernandez, Sandra E., Sernia, Conrad and Bradley, Adrian J. (2016) Adrenocortical function in cane toads from different environments. Comparative Biochemistry and Physiology Part A : Molecular and Integrative Physiology, 195 65-72. doi:10.1016/j.cbpa.2016.02.001


Author Hernandez, Sandra E.
Sernia, Conrad
Bradley, Adrian J.
Title Adrenocortical function in cane toads from different environments
Journal name Comparative Biochemistry and Physiology Part A : Molecular and Integrative Physiology   Check publisher's open access policy
ISSN 1531-4332
1095-6433
Publication date 2016-05-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.cbpa.2016.02.001
Open Access Status Not Open Access
Volume 195
Start page 65
End page 72
Total pages 8
Place of publication Philadelphia, PA United States
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
The adrenocortical function of cane toads (Rhinella marina) exposed to different experimental procedures, as well as captured from different environments, was assessed by challenging the hypothalamic–pituitary–adrenal (HPA) axis. It was found that restriction stress as well as cannulation increased plasma corticosterone (B) levels for up to 12 h. A single dose of dexamethasone (DEX 2 mg/kg) significantly reduced B levels demonstrating its potential for use in the evaluation of the HPA axis in amphibia. We also demonstrate that 0.05 IU/g BW (im) of synthetic adrenocorticotropic hormone (ACTH) significantly increased plasma B levels in cane toads. Changes in size area of the cortical cells were positively associated with total levels of B after ACTH administration. We also found differences in adrenal activity between populations. This was assessed by a DEX-ACTH test. The animals captured from the field and maintained in captivity for one year at the animal house (AH) present the highest levels of total and free B after ACTH administration. We also found that animals from the front line of dispersion in Western Australia (WA) present the weakest adrenal response to a DEX-ACTH test. The animals categorized as long established in Queensland Australia (QL), and native in Mexico (MX), do not shown a marked difference in the HPA activity. Finally we found that in response to ACTH administration, females reach significantly higher levels of plasma B than males. For the first time the adrenocortical response in cane toads exposed to different experimental procedures, as well as from different populations was assessed systematically.
Keyword Rhinella marina
Amphibia
Stress response
ACTH
Dexamethasone
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biomedical Sciences Publications
 
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