A rapid phenotyping method for adult plant resistance to leaf rust in wheat

Riaz, Adnan, Periyannan, Sambasivam, Aitken, Elizabeth and Hickey, Lee (2016) A rapid phenotyping method for adult plant resistance to leaf rust in wheat. Plant Methods, 12 17: 1-10. doi:10.1186/s13007-016-0117-7

Author Riaz, Adnan
Periyannan, Sambasivam
Aitken, Elizabeth
Hickey, Lee
Title A rapid phenotyping method for adult plant resistance to leaf rust in wheat
Journal name Plant Methods   Check publisher's open access policy
ISSN 1746-4811
Publication date 2016-03-02
Year available 2016
Sub-type Article (original research)
DOI 10.1186/s13007-016-0117-7
Open Access Status DOI
Volume 12
Issue 17
Start page 1
End page 10
Total pages 10
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2017
Language eng
Formatted abstract
Leaf rust (LR), caused by Puccinia triticina and is an important disease of wheat (Triticum aestivum L.). The most sustainable method for controlling rust diseases is deployment of cultivars incorporating adult plant resistance (APR). However, phenotyping breeding populations or germplasm collections for resistance in the field is dependent on weather conditions and limited to once a year. In this study, we explored the ability to phenotype APR to LR under accelerated growth conditions (AGC; i.e. constant light and controlled temperature) using a method that integrates assessment at both seedling and adult growth stages. A panel of 21 spring wheat genotypes, including disease standards carrying known APR genes (i.e. Lr34 and Lr46) were characterised under AGC and in the field.

Disease response displayed by adult wheat plants grown under AGC (i.e. flag-2 leaf) was highly correlated with field-based measures (R2 = 0.77). The integrated method is more efficient—requiring less time, space, and labour compared to traditional approaches that perform seedling and adult plant assays separately. Further, this method enables up to seven consecutive adult plant LR assays compared to one in the field.

The integrated seedling and adult plant phenotyping method reported in this study provides a great tool for identifying APR to LR. Assessing plants at early growth stages can enable selection for desirable gene combinations and crossing of the selected plants in the same plant generation. The method has the potential to be scaled-up for screening large numbers of fixed lines and segregating populations. This strategy would reduce the time required for moving APR genes into adapted germplasm or combining traits in top crosses in breeding programs. This method could accelerate selection for resistance factors effective across diverse climates by conducting successive cycles of screening performed at different temperature regimes.
Keyword Wheat
Leaf rust
Puccinia triticina
Adult plant resistance
Accelerated growth conditions
Disease screening
Wheat breeding
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
Queensland Alliance for Agriculture and Food Innovation
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Created: Mon, 14 Mar 2016, 11:56:28 EST by Lee Hickey on behalf of Centre for Plant Science