Triggered exocytosis of the protozoan Tetrahymena as a source of bioflocculation and a controllable dewatering method for efficient harvest of microalgal cultures

Jakob, Gisela, Stephens, Evan, Feller, Rafael, Oey, Melanie, Hankamer, Ben and Ross, Ian L. (2016) Triggered exocytosis of the protozoan Tetrahymena as a source of bioflocculation and a controllable dewatering method for efficient harvest of microalgal cultures. Algal Research, 13 148-158. doi:10.1016/j.algal.2015.11.011


Author Jakob, Gisela
Stephens, Evan
Feller, Rafael
Oey, Melanie
Hankamer, Ben
Ross, Ian L.
Title Triggered exocytosis of the protozoan Tetrahymena as a source of bioflocculation and a controllable dewatering method for efficient harvest of microalgal cultures
Formatted title
Triggered exocytosis of the protozoan Tetrahymena as a source of bioflocculation and a controllable dewatering method for efficient harvest of microalgal cultures
Journal name Algal Research   Check publisher's open access policy
ISSN 2211-9264
Publication date 2016-01
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.algal.2015.11.011
Open Access Status Not Open Access
Volume 13
Start page 148
End page 158
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2016
Language eng
Formatted abstract
Microalgae offer a promising route to the production of high-value products, foods, animal feeds, and biofuels. Efficient algal dewatering strategies are therefore important for minimising energy and costs. Bio-flocculation is a potentially low-cost, low-energy harvesting strategy which can be facilitated by the microbial production of extracellular polymeric substances (EPS), but must be inducible since productive algal culture generally requires cells to be in suspension. Here we show that algal bio-flocculation can be controllably induced using the protozoan Tetrahymena. As little as 1:400 starved Tetrahymena to algal cells can be used to initiate rapid bio-flocculation. We demonstrate that stimulators of ryanodine receptors (caffeine and p-chlorocresol) trigger exocytosis in Tetrahymena and that inexpensive physicochemical stimuli (ammonium ions, shifts in pH and salinity) can also be effective. We suggest that triggered EPS secretion by protozoans in the starved state may explain apparently spontaneous bioflocculation of algae in both natural and artificial systems.
Keyword Microalgae
Bioflocculation
Exocytosis
Protozoa
Tetrahymena
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Published online 10 December 2015

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 13 Mar 2016, 00:26:21 EST by System User on behalf of Learning and Research Services (UQ Library)