Cerebral microcirculation during experimental normovolaemic anemia

Bellapart, Judith, Cuthbertson, Kylie, Dunster, Kimble, Diab, Sara, Platts, David G., Raffel, O. Christopher, Gabrielian, Levon, Barnett, Adrian, Paratz, Jenifer, Boots, Rob and Fraser, John F. (2016) Cerebral microcirculation during experimental normovolaemic anemia. Frontiers in Neurology, 7 FEB: . doi:10.3389/fneur.2016.00006

Author Bellapart, Judith
Cuthbertson, Kylie
Dunster, Kimble
Diab, Sara
Platts, David G.
Raffel, O. Christopher
Gabrielian, Levon
Barnett, Adrian
Paratz, Jenifer
Boots, Rob
Fraser, John F.
Title Cerebral microcirculation during experimental normovolaemic anemia
Journal name Frontiers in Neurology   Check publisher's open access policy
ISSN 1664-2295
Publication date 2016-02
Sub-type Article (original research)
DOI 10.3389/fneur.2016.00006
Open Access Status DOI
Volume 7
Issue FEB
Total pages 9
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Collection year 2017
Language eng
Formatted abstract
Anemia is accepted among critically ill patients as an alternative to elective blood transfusion. This practice has been extrapolated to head injury patients with only one study comparing the effects of mild anemia on neurological outcome. There are no studies quantifying microcirculation during anemia. Experimental studies suggest that anemia leads to cerebral hypoxia and increased rates of infarction, but the lack of clinical equipoise, when testing the cerebral effects of transfusion among critically injured patients, supports the need of experimental studies. The aim of this study was to quantify cerebral microcirculation and the potential presence of axonal damage in an experimental model exposed to normovolaemic anemia, with the intention of describing possible limitations within management practices in critically ill patients. Under non-recovered anesthesia, six Merino sheep were instrumented using an intracardiac transeptal catheter to inject coded microspheres into the left atrium to ensure systemic and non-chaotic distribution. Cytometric analyses quantified cerebral microcirculation at specific regions of the brain. Amyloid precursor protein staining was used as an indicator of axonal damage. Animals were exposed to normovolaemic anemia by blood extractions from the indwelling arterial catheter with simultaneous fluid replacement through a venous central catheter. Simultaneous data recording from cerebral tissue oxygenation, intracranial pressure, and cardiac output was monitored. A regression model was used to examine the effects of anemia on microcirculation with a mixed model to control for repeated measures. Homogeneous and normal cerebral microcirculation with no evidence of axonal damage was present in all cerebral regions, with no temporal variability, concluding that acute normovolaemic anemia does not result in short-term effects on cerebral microcirculation in the ovine brain.
Keyword Anemia
APP staining
Traumatic Brain-Injury
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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Sub-type: Article (original research)
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