The apoptotic engulfment machinery regulates axonal degeneration in C. elegans neurons

Nichols, Annika L.A., Meelkop, Ellen, Linton, Casey, Giordano-Santini, Rosina, Sullivan, Robert K., Donato, Alessandra, Nolan, Cara, Hall, David H., Xue, Ding, Neumann, Brent and Hilliard, Massimo A. (2016) The apoptotic engulfment machinery regulates axonal degeneration in C. elegans neurons. Cell Reports, 14 7: 1673-1683. doi:10.1016/j.celrep.2016.01.050


Author Nichols, Annika L.A.
Meelkop, Ellen
Linton, Casey
Giordano-Santini, Rosina
Sullivan, Robert K.
Donato, Alessandra
Nolan, Cara
Hall, David H.
Xue, Ding
Neumann, Brent
Hilliard, Massimo A.
Title The apoptotic engulfment machinery regulates axonal degeneration in C. elegans neurons
Formatted title
The apoptotic engulfment machinery regulates axonal degeneration in C. elegans neurons
Journal name Cell Reports   Check publisher's open access policy
ISSN 2211-1247
Publication date 2016-02-23
Sub-type Article (original research)
DOI 10.1016/j.celrep.2016.01.050
Open Access Status DOI
Volume 14
Issue 7
Start page 1673
End page 1683
Total pages 11
Place of publication New York, United States
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Axonal degeneration is a characteristic feature of neurodegenerative disease and nerve injury. Here, we characterize axonal degeneration in Caenorhabditis elegans neurons following laser-induced axotomy. We show that this process proceeds independently of the WLDS and Nmnat pathway and requires the axonal clearance machinery that includes the conserved transmembrane receptor CED-1/Draper, the adaptor protein CED-6, the guanine nucleotide exchange factor complex Crk/Mbc/dCed-12 (CED-2/CED-5/CED-12), and the small GTPase Rac1 (CED-10). We demonstrate that CED-1 and CED-6 function non-cell autonomously in the surrounding hypodermis, which we show acts as the engulfing tissue for the severed axon. Moreover, we establish a function in this process for CED-7, an ATP-binding cassette (ABC) transporter, and NRF-5, a lipid-binding protein, both associated with release of lipid-vesicles during apoptotic cell clearance. Thus, our results reveal the existence of a WLDS/Nmnat-independent axonal degeneration pathway, conservation of the axonal clearance machinery, and a function for CED-7 and NRF-5 in this process.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Centre for Ageing Dementia Research Publications
 
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Created: Fri, 11 Mar 2016, 16:12:30 EST by Susan Day on behalf of Learning and Research Services (UQ Library)