Inactivation of dengue, chikungunya, and Ross River viruses in platelet concentrates after treatment with ultraviolet C light

Faddy, Helen M., Fryk, Jesse J., Prow, Natalie A., Watterson, Daniel, Young, Paul R., Hall, Roy A., Tolksdorf, Frank, Sumian, Chryslain, Gravemann, Ute, Seltsam, Axel and Marks, Denese C. (2016) Inactivation of dengue, chikungunya, and Ross River viruses in platelet concentrates after treatment with ultraviolet C light. Transfusion, 56 6pt2: 1548-1555. doi:10.1111/trf.13519


Author Faddy, Helen M.
Fryk, Jesse J.
Prow, Natalie A.
Watterson, Daniel
Young, Paul R.
Hall, Roy A.
Tolksdorf, Frank
Sumian, Chryslain
Gravemann, Ute
Seltsam, Axel
Marks, Denese C.
Title Inactivation of dengue, chikungunya, and Ross River viruses in platelet concentrates after treatment with ultraviolet C light
Journal name Transfusion   Check publisher's open access policy
ISSN 1537-2995
0041-1132
Publication date 2016-06-01
Year available 2016
Sub-type Article (original research)
DOI 10.1111/trf.13519
Open Access Status Not Open Access
Volume 56
Issue 6pt2
Start page 1548
End page 1555
Total pages 8
Place of publication Hoboken, NJ United States
Publisher Wiley-Blackwell Publishing
Collection year 2017
Language eng
Formatted abstract
BACKGROUND
Arboviruses, including dengue (DENV 1-4), chikungunya (CHIKV), and Ross River (RRV), are emerging viruses that are a risk for transfusion safety globally. An approach for managing this risk is pathogen inactivation, such as the THERAFLEX UV-Platelets system. We investigated the ability of this system to inactivate the above mentioned arboviruses.

STUDY DESIGN AND METHODS
DENV 1-4, CHIKV, or RRV were spiked into buffy coat (BC)-derived platelet (PLT) concentrates in additive solution and treated with the THERAFLEX UV-Platelets system at the following doses: 0.05, 0.1, 0.15, and 0.2 J/cm2 (standard dose). Pre- and posttreatment samples were taken for each dose, and the level of viral infectivity was determined.

RESULTS
At the standard ultraviolet C (UVC) dose (0.2 J/cm2), viral inactivation of at least 4.43, 6.34, and 5.13 log or more, was observed for DENV 1-4, CHIKV, and RRV, respectively. A dose dependency in viral inactivation was observed with increasing UVC doses.

CONCLUSIONS
Our study has shown that DENV, CHIKV, and RRV, spiked into BC-derived PLT concentrates, were inactivated by the THERAFLEX UV-Platelets system to the limit of detection of our assay, suggesting that this system could contribute to the safety of PLT concentrates with respect to these emerging arboviruses.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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Created: Fri, 11 Mar 2016, 12:44:07 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences