Cell polarity defines three distinct domains in pancreatic beta cells

Gan, Wan J., Zavortink, Michael, Ludick, Christine, Templin, Rachel, Webb, Robyn, Webb, Richard, Ma, Wei, Poronnik, Philip, Parton, Robert G., Gaisano, Herbert Y., Shewan, Annette M. and Thorn, Peter (2016) Cell polarity defines three distinct domains in pancreatic beta cells. Journal of Cell Science, 1-9. doi:10.1242/jcs.185116


Author Gan, Wan J.
Zavortink, Michael
Ludick, Christine
Templin, Rachel
Webb, Robyn
Webb, Richard
Ma, Wei
Poronnik, Philip
Parton, Robert G.
Gaisano, Herbert Y.
Shewan, Annette M.
Thorn, Peter
Title Cell polarity defines three distinct domains in pancreatic beta cells
Formatted title
Cell polarity defines three distinct domains in pancreatic β-cells
Journal name Journal of Cell Science   Check publisher's open access policy
ISSN 0021-9533
1477-9137
Publication date 2016-03-18
Sub-type Article (original research)
DOI 10.1242/jcs.185116
Open Access Status DOI
Start page 1
End page 9
Total pages 9
Place of publication Cambridge, United Kingdom
Publisher The Company of Biologists
Collection year 2017
Language eng
Formatted abstract
The structural organisation of pancreatic β-cells in the islets of Langerhans is relatively unknown. Here, using three-dimensional (3D) two-photon, 3D confocal and 3D block-face serial electron microscopy, we demonstrate a consistent in situ polarisation of β-cells and define three distinct cell surface domains. An apical domain located at the vascular apogee of β-cells, defined by the location of PAR-3 (also known as PARD3) and ZO-1 (also known as TJP1), delineates an extracellular space into which adjacent β-cells project their primary cilia. A separate lateral domain, is enriched in scribble and Dlg, and colocalises with E-cadherin and GLUT2 (also known as SLC2A2). Finally, a distinct basal domain, where the β-cells contact the islet vasculature, is enriched in synaptic scaffold proteins such as liprin. This 3D analysis of β-cells within intact islets, and the definition of distinct domains, provides new insights into understanding β-cell structure and function.
Keyword Insulin
Polarity
Islet
Diabetes
β-Cell
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Fri, 11 Mar 2016, 11:53:23 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences