Exploring the transcriptomic data of the Australian paralysis tick, Ixodes holocyclus

Ong, C. T., Rodriguez-Valle, M., Moolhuijzen, P. M., Barrero, R. A., Hunter, A., Szabo, T., Bellgard, M. I. and Lew-Tabor, A. E. (2016) Exploring the transcriptomic data of the Australian paralysis tick, Ixodes holocyclus. GSTF Journal of Veterinary Science (JVet), 3 1: . doi:10.7603/s40871-016-0001-y


Author Ong, C. T.
Rodriguez-Valle, M.
Moolhuijzen, P. M.
Barrero, R. A.
Hunter, A.
Szabo, T.
Bellgard, M. I.
Lew-Tabor, A. E.
Title Exploring the transcriptomic data of the Australian paralysis tick, Ixodes holocyclus
Formatted title
Exploring the transcriptomic data of the Australian paralysis tick, Ixodes holocyclus
Journal name GSTF Journal of Veterinary Science (JVet)
ISSN 2345-7910
2345-7880
Publication date 2016-03
Year available 2016
Sub-type Article (original research)
DOI 10.7603/s40871-016-0001-y
Open Access Status DOI
Volume 3
Issue 1
Total pages 10
Place of publication Singapore, Singapore
Publisher Global Science and Technology Forum
Collection year 2017
Language eng
Formatted abstract
Ixodes holocyclus is the paralysis tick commonly found in Australia. I. holocyclus does not cause paralysis in the primary host – bandicoots, but markedly affects secondary hosts such as companion animals, livestock and humans. Holocyclotoxins are the neurotoxin molecules in I. holocyclus responsible for paralysis symptoms. There is a limited understanding of holocyclotoxins due to the difficulties in purifying and expressing these toxins in vitro. Next-generation sequencing technologies were utilised for the first time to generate transcriptome data from two cDNA samples –salivary glands samples collected from female adult ticks engorged on paralysed companion animals and on bandicoots. Contig-encoded proteins in each library were annotated according to their best BLAST match against several databases and functionally assigned into six protein categories: housekeeping, transposable elements, pathogen-related, hypothetical, secreted and novel. The “secreted protein” category is comprised of ten protein families: enzymes, protease inhibitors, antigens, mucins, immunity-related, lipocalins, glycine-rich, putative secreted, salivary and toxin-like. Comparisons of contig representation between the two libraries reveal the differential expression of tick proteins collected from different hosts. This study provides a preliminary description of the I. holocyclus tick salivary gland transcriptome.
Keyword Paralysis tick
Ixodes holocyclus
I. holocyclus
BLAST
Enzymes
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Agriculture and Food Innovation
 
Versions
Version Filter Type
Citation counts: Google Scholar Search Google Scholar
Created: Tue, 08 Mar 2016, 11:36:54 EST by Dr Manuel Rodriguez Valle on behalf of Centre for Animal Science