Microarray analysis of gene expression in germinating barley embryos (Hordeum vulgare L.)

Watson, Loraine and Henry, Robert J. (2005) Microarray analysis of gene expression in germinating barley embryos (Hordeum vulgare L.). Functional and Integrative Genomics, 5 3: 155-162. doi:10.1007/s10142-005-0133-6


Author Watson, Loraine
Henry, Robert J.
Title Microarray analysis of gene expression in germinating barley embryos (Hordeum vulgare L.)
Formatted title
Microarray analysis of gene expression in germinating barley embryos (Hordeum vulgare L.)
Journal name Functional and Integrative Genomics   Check publisher's open access policy
ISSN 1438-793X
1438-7948
Publication date 2005-07-01
Sub-type Article (original research)
DOI 10.1007/s10142-005-0133-6
Volume 5
Issue 3
Start page 155
End page 162
Total pages 8
Place of publication Heidelberg, Germany
Publisher Springer
Formatted abstract
A cDNA library containing approximately 5,000 clones from germinating barley embryos was constructed and used to examine the variation in gene expression patterns during the first 4 days postimbibition. The expression profiles of embryos (including scutellum) from 4 to 96 h postimbibition were compared to a reference profile from 24 h postimbibition using microarray analysis. A subset of clones exhibiting tenfold or greater differential expression patterns was sequenced to elucidate function. All of the sequenced clones could be identified to at least EST level with 64% exhibiting homology to published protein sequences. Almost 95% of the library exhibited similar expression levels at the 4 h time point as at the 24 h reference point. From 24 to 96 h, however, considerable fluctuations in gene expression occurred. The observed patterns of gene expression for the classified genes are consistent with the expected genetic changes required to prepare an embryo for germinative development. A replicate set of clones for the 23-kDa jasmonate-induced protein was identified. The current data not only provides conclusive evidence for the expression patterns of this abundant stress-response protein in germinating embryos, but also serves to validate previous research into JIP-23 isoforms, function and the relationship between timing of mRNA upregulation and protein abundance.
Keyword Barley embryo
Germination
JIP-23
Microarray analysis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Nutrition and Food Sciences Publications
 
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