Enhanced ribosomal association of p27Kip1 mRNA is a mechanism contributing to accumulation during growth arrest

Millard, S. Sean, Yan, Jie Shi, Nguyen, Hoang, Pagano, Michele, Kiyokawa, Hiroaki and Koff, Andrew (1997) Enhanced ribosomal association of p27Kip1 mRNA is a mechanism contributing to accumulation during growth arrest. Journal of Biological Chemistry, 272 11: 7093-7098. doi:10.1074/jbc.272.11.7093

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ381324_OA.pdf Full text (open access) application/pdf 387.44KB 0

Author Millard, S. Sean
Yan, Jie Shi
Nguyen, Hoang
Pagano, Michele
Kiyokawa, Hiroaki
Koff, Andrew
Title Enhanced ribosomal association of p27Kip1 mRNA is a mechanism contributing to accumulation during growth arrest
Formatted title
Enhanced ribosomal association of p27Kip1 mRNA is a mechanism contributing to accumulation during growth arrest
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 1083-351X
0021-9258
Publication date 1997-03-14
Sub-type Article (original research)
DOI 10.1074/jbc.272.11.7093
Open Access Status File (Publisher version)
Volume 272
Issue 11
Start page 7093
End page 7098
Total pages 6
Place of publication Rockville, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
p27Kip1 regulates the decision to enter into S-phase or withdraw from the cell cycle by establishing an inhibitory threshold above which G1 cyclin-dependent kinases accumulate before activation. We have used the HL-60 cell line to study regulation of p27 as cells withdraw from the cell cycle following treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). We found that the amount of p27 is maximal in G0 cells, lower in G1 cells, and undetectable in S-phase cells. In contrast to the protein, the amount of p27 mRNA was the same in these populations, suggesting that accumulation of p27 during the cell cycle and as cells withdraw from the cell cycle is controlled by post-transcriptional mechanisms. In S-phase cells, the degradation of p27 appears to predominate as a regulatory mechanism. In G0 cells, there was an increase in the synthesis rate of p27. Our data demonstrate that, in G0 cells, accumulation of p27 is due to an increase in the amount of p27 mRNA in polyribosomes.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 164 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 167 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 04 Mar 2016, 10:45:56 EST by Ms Joanne Biles on behalf of School of Biomedical Sciences