A U-rich element in the 5′ untranslated region is necessary for the translation of p27 mRNA

Millard, S. Sean, Vidal, Anxo, Markus, Maurice and Koff, Andrew (2000) A U-rich element in the 5′ untranslated region is necessary for the translation of p27 mRNA. Molecular and Cellular Biology, 20 16: 5947-5959. doi:10.1128/MCB.20.16.5947-5959.2000

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Author Millard, S. Sean
Vidal, Anxo
Markus, Maurice
Koff, Andrew
Title A U-rich element in the 5′ untranslated region is necessary for the translation of p27 mRNA
Journal name Molecular and Cellular Biology   Check publisher's open access policy
ISSN 0270-7306
Publication date 2000-08
Sub-type Article (original research)
DOI 10.1128/MCB.20.16.5947-5959.2000
Open Access Status File (Publisher version)
Volume 20
Issue 16
Start page 5947
End page 5959
Total pages 13
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Increased translation of p27 mRNA correlates with withdrawal of cells from the cell cycle. This raised the possibility that antimitogenic signals might mediate their effects on p27 expression by altering complexes that formed on p27 mRNA, regulating its translation. In this report, we identify a U-rich sequence in the 5′ untranslated region (5′UTR) of p27 mRNA that is necessary for efficient translation in proliferating and nonproliferating cells. We show that a number of factors bind to the 5′UTR in vitro in a manner dependent on the U-rich element, and their availability in the cytosol is controlled in a growth- and cell cycle-dependent fashion. One of these factors is HuR, a protein previously implicated in mRNA stability, transport, and translation. Another is hnRNP C1 and C2, proteins implicated in mRNA processing and the translation of a specific subset of mRNAs expressed in differentiated cells. In lovastatin-treated MDA468 cells, the mobility of the associated hnRNP C1 and C2 proteins changed, and this correlated with increased p27 expression. Together, these data suggest that the U-rich dependent RNP complex on the 5′UTR may regulate the translation of p27 mRNA and may be a target of antimitogenic signals.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 104 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 04 Mar 2016, 10:12:15 EST by Ms Joanne Biles on behalf of School of Biomedical Sciences