Expression of ABA synthesis and metabolism genes under different irrigation strategies and atmospheric VPDs is associated with stomatal conductance in grapevine (Vitis vinifera L. cv Cabernet Sauvignon)

Speirs, Jim, Binney, Allan, Collins, Marisa, Edwards, Everard and Loveys, Brian (2013) Expression of ABA synthesis and metabolism genes under different irrigation strategies and atmospheric VPDs is associated with stomatal conductance in grapevine (Vitis vinifera L. cv Cabernet Sauvignon). Journal of Experimental Botany, 64 7: 1907-1916. doi:10.1093/jxb/ert052


Author Speirs, Jim
Binney, Allan
Collins, Marisa
Edwards, Everard
Loveys, Brian
Title Expression of ABA synthesis and metabolism genes under different irrigation strategies and atmospheric VPDs is associated with stomatal conductance in grapevine (Vitis vinifera L. cv Cabernet Sauvignon)
Formatted title
Expression of ABA synthesis and metabolism genes under different irrigation strategies and atmospheric VPDs is associated with stomatal conductance in grapevine (Vitis vinifera L. cv Cabernet Sauvignon)
Journal name Journal of Experimental Botany   Check publisher's open access policy
ISSN 0022-0957
1460-2431
Publication date 2013-04
Sub-type Article (original research)
DOI 10.1093/jxb/ert052
Open Access Status Not Open Access
Volume 64
Issue 7
Start page 1907
End page 1916
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract
The influence of different levels of irrigation and of variation in atmospheric vapour pressure deficit (VPD) on the synthesis, metabolism, and transport of abscisic acid (ABA) and the effects on stomatal conductance were examined in field-grown Cabernet Sauvignon grapevines. Xylem sap, leaf tissue, and root tissue were collected at regular intervals during two seasons in conjunction with measurements of leaf water potential (ψleaf) and stomatal conductance (gs). The different irrigation levels significantly altered the ψleaf and gs of the vines across both seasons. ABA abundance in the xylem sap was correlated with g s. The expression of genes associated with ABA synthesis, NCED1 and NCED2, was higher in the roots than in the leaves throughout and highest in the roots in mid January, a time when soil moisture declined and VPD was at its highest. Their expression in roots was also inversely related to the levels of irrigation and correlated with ABA abundance in the roots, xylem sap, and leaves. Three genes encoding ABA 8′-hydroxylases were isolated and their identities confirmed by expression in yeast cells. The expression of one of these, Hyd1, was elevated in leaves when VPD was below 2.0-2.5 kPa and minimal at higher VPD levels. The results provide evidence that ABA plays an important role in linking stomatal response to soil moisture status and that changes in ABA catabolism at or near its site of action allows optimization of gas exchange to current environmental conditions.
Keyword Abscisic acid
ABA
ABA 8′-hydroxylase genes
Grapevine
Irrigation
NCED genes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Tue, 01 Mar 2016, 10:00:51 EST by Marisa Collins on behalf of School of Agriculture and Food Sciences