Antibacterial low molecular weight cationic polymers: Dissecting the contribution of hydrophobicity, chain length and charge to activity

Grace, James L., Huang, Johnny X., Cheah, Soon-Ee, Truong, Nghia P., Cooper, Matthew A., Li, Jian, Davis, Thomas P., Quinn, John F., Velkov, Tony and Whittaker, Michael R. (2016) Antibacterial low molecular weight cationic polymers: Dissecting the contribution of hydrophobicity, chain length and charge to activity. RSC Advances, 6 19: 15469-15477. doi:10.1039/c5ra24361k


Author Grace, James L.
Huang, Johnny X.
Cheah, Soon-Ee
Truong, Nghia P.
Cooper, Matthew A.
Li, Jian
Davis, Thomas P.
Quinn, John F.
Velkov, Tony
Whittaker, Michael R.
Title Antibacterial low molecular weight cationic polymers: Dissecting the contribution of hydrophobicity, chain length and charge to activity
Journal name RSC Advances   Check publisher's open access policy
ISSN 2046-2069
Publication date 2016
Sub-type Article (original research)
DOI 10.1039/c5ra24361k
Volume 6
Issue 19
Start page 15469
End page 15477
Total pages 9
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Collection year 2017
Language eng
Formatted abstract
The balance of cationicity and hydrophobicity can profoundly affect the performance of antimicrobial polymers. To this end a library of 24 cationic polymers with uniquely low degrees of polymerization was synthesized via Cu(0)-mediated polymerization, using three different cationic monomers and two initiators: providing two different hydrocarbon chain tail lengths (C2 and C12). The polymers exhibited structure-dependent antibacterial activity when tested against a selection of bacteria, viz., Staphylococcus aureus ATCC 29213, Klebsiella pneumoniae ATCC 13883, Acinetobacter baumannii ATCC 19606, and Pseudomonas aeruginosa ATCC 27853 as a representative palette of Gram-positive and Gram-negative ESKAPE pathogens. The five best-performing polymers were identified for additional testing against the polymyxin-resistant A. baumannii ATCC 19606R strain. Polymers having the lowest DP and a C12 hydrophobic tail were shown to provide the broadest antimicrobial activity against the bacteria panel studied as evidenced by lower minimum inhibitory concentrations (MICs). An optimal polymer composition was identified, and its mechanism of action investigated via membrane permeability testing against Escherichia coli. Membrane disruption was identified as the most probable mechanism for bacteria cell killing.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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