Ovine platelet function is unaffected by extracorporeal membrane oxygenation within the first 24 h

Hayes, Rylan A., Foley, Samuel, Shekar, Kiran, Diab, Sara, Dunster, Kimble R., McDonald, Charles and Fraser, John F. (2015) Ovine platelet function is unaffected by extracorporeal membrane oxygenation within the first 24 h. Blood Coagulation and Fibrinolysis, 26 7: 816-822. doi:10.1097/MBC.0000000000000360


Author Hayes, Rylan A.
Foley, Samuel
Shekar, Kiran
Diab, Sara
Dunster, Kimble R.
McDonald, Charles
Fraser, John F.
Title Ovine platelet function is unaffected by extracorporeal membrane oxygenation within the first 24 h
Journal name Blood Coagulation and Fibrinolysis   Check publisher's open access policy
ISSN 0957-5235
1473-5733
Publication date 2015-10
Year available 2015
Sub-type Article (original research)
DOI 10.1097/MBC.0000000000000360
Open Access Status Not Open Access
Volume 26
Issue 7
Start page 816
End page 822
Total pages 7
Place of publication Philadelphia, PA United States
Publisher Lippincott Williams and Wilkins
Collection year 2016
Language eng
Formatted abstract
This study investigated platelet dysfunction during short-term extracorporeal membrane oxygenation (ECMO) and secondarily to determine if hyperoxaemia contributes to this dysfunction. Healthy sheep were anaesthetized and maintained on ECMO for either 2 or 24 h, with or without induction of smoke inhalation acute lung injury. A specialized animal-operating theatre was used to conduct the experimentation. Forty-three healthy female sheep were randomized into either a test or a control group. Following anaesthesia, test groups received ECMO ± smoke inhalation acute lung injury (SALI), whereas control groups were maintained with ventilation only ± SALI. Physiological, biochemical and coagulation data were obtained throughout via continuous monitoring and blood sampling. Platelet function was quantified through whole blood impedance aggregometry using Multiplate. Ovine platelet activity induced by adenosine diphosphate (ADP) and collagen was unaffected during the first 24 h of ECMO. However, progressive divergence of ADP-induced platelet activity was noted at cessation of the experiment. PaO2 was inversely related to ADP-dependent platelet activity in the ECMO groups – a relationship not identified in the control groups. ADP and collagen-dependent platelet activity are not significantly affected within the first 24 h of ECMO in sheep. However, dysfunction in ADP-dependent platelet activity may have continued to develop if observed beyond 24 h. Hyperoxaemia during ECMO does appear to affect how platelets react to ADP and may contribute to this developing dysfunction. Long-term animal models and investigation in clinical animals are warranted to fully investigate platelet function during ECMO.
Keyword Acute lung injury
Extracorporeal membrane oxygenation
Hyperoxaemia
Platelet
Thrombosis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
 
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