Virus-derived vectors for the expression of multiple proteins in plants

Saxena, Pooja, Thuenemann, Eva C., Sainsbury, Frank and Lomonossoff, George P. (2016). Virus-derived vectors for the expression of multiple proteins in plants. In MacDonald, Jacqueline, Kolotilin, Igor and Menassa, Rima (Ed.), Recombinant proteins from plants: methods and protocols 2nd ed. (pp. 39-54) New York, NY United States: Springer New York. doi:10.1007/978-1-4939-3289-4_3


Author Saxena, Pooja
Thuenemann, Eva C.
Sainsbury, Frank
Lomonossoff, George P.
Title of chapter Virus-derived vectors for the expression of multiple proteins in plants
Title of book Recombinant proteins from plants: methods and protocols
Place of Publication New York, NY United States
Publisher Springer New York
Publication Year 2016
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-4939-3289-4_3
Open Access Status Not yet assessed
Year available 2016
Series Methods in molecular biology
Edition 2nd
ISBN 9781493932887
9781493932894
ISSN 1064-3745
Editor MacDonald, Jacqueline
Kolotilin, Igor
Menassa, Rima
Volume number 1385
Chapter number 3
Start page 39
End page 54
Total pages 16
Total chapters 19
Collection year 2017
Language eng
Formatted Abstract/Summary
This chapter constitutes a practical guide to using the “pEAQ” vector series for transient or stable expression of one or more protein(s) in Nicotiana benthamiana plants. The pEAQ vectors are a series of small binary vectors designed for controlled expression of multiple proteins in plants. To achieve high levels of expression, an expression system based on translational enhancement by the untranslated regions of RNA-2 from cowpea mosaic virus (CPMV), named CPMV-HT, is used. The expression vector pEAQ-HT combines the user-friendly pEAQ plasmid with CPMV-HT to provide a system for high-level expression of proteins in plants.
Keyword Transient expression
Agro-infiltration
Viral vectors
pEAQ
CMPV-Ht
Q-Index Code BX
Q-Index Status Provisional Code
Institutional Status UQ

 
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