Short- and long-term tracking of anionic ultrasmall nanoparticles in kidney

Liang, Xiaowen, Wang, Haolu, Zhu, Yian, Zhang, Run, Cogger, Victoria C., Liu, Xin, Xu, Zhi Ping, Grice, Jeffrey E. and Roberts, Michael S. (2016) Short- and long-term tracking of anionic ultrasmall nanoparticles in kidney. Acs Nano, 10 1: 387-395. doi:10.1021/acsnano.5b05066


Author Liang, Xiaowen
Wang, Haolu
Zhu, Yian
Zhang, Run
Cogger, Victoria C.
Liu, Xin
Xu, Zhi Ping
Grice, Jeffrey E.
Roberts, Michael S.
Title Short- and long-term tracking of anionic ultrasmall nanoparticles in kidney
Journal name Acs Nano   Check publisher's open access policy
ISSN 1936-0851
1936-086X
Publication date 2016-01
Year available 2016
Sub-type Article (original research)
DOI 10.1021/acsnano.5b05066
Open Access Status Not Open Access
Volume 10
Issue 1
Start page 387
End page 395
Total pages 9
Place of publication Washington DC, United States
Publisher American Chemical Society
Collection year 2017
Language eng
Formatted abstract
While biodistribution of nanoparticles (NPs) has been widely studied at the organ level, relatively little is known about their disposition in organs at the cellular level, especially after long-term exposure. The kidney is regarded as the key organ for the clearance of ultrasmall NPs (<5.5 nm). However, recent studies indicate that NPs in this size range could accumulate in the kidney for extended times without urinary excretion. Using negatively charged quantum dots (QDs) (∼3.7 nm) as a model system, we examined the suborgan disposition of anionic ultrasmall NPs in the kidney at the cellular level after intravenous injection by multiphoton microscopy coupled with fluorescence lifetime imaging. Most of the NPs were initially distributed in the peritubular capillaries or glomerular arterioles after injection, whereas they passed through the fenestrated glomerular endothelium and were gradually taken up by mesangial cells up to 30 days after injection. Only trace amounts of anionic QDs could be detected in the urine, which could be attributed to the barrier of the anionic glomerular basement membrane preventing filtration of anionic QDs. In contrast, cationic QDs of similar size (∼5.67 nm) were found to be readily excreted into urine. This study thus highlights the importance of surface charge in determining renal clearance of ultrasmall NPs. It provides a framework for characterizing and predicting the subcellular disposition in organs and long-term targeting of other NPs, with a physiologically based kinetic model being subsequently developed to describe the suborgan kinetics of anionic ultrasmall NPs.
Keyword Ultrasmall nanoparticles
Renal disposition
Suborgan kinetics
surface charge
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 21 Feb 2016, 00:20:50 EST by System User on behalf of Learning and Research Services (UQ Library)