Intravital multiphoton imaging of the kidney: tubular structure and metabolism

Small, David M., Sanchez, Washington Y. and Gobe, Glenda C. (2016). Intravital multiphoton imaging of the kidney: tubular structure and metabolism. In Tim D. Hewitson, Edward R. Smith and Stephen G. Holt (Ed.), Kidney research: experimental protocols Second edition ed. (pp. 155-172) New York, NY, United States: Humana Press. doi:10.1007/978-1-4939-3353-2_12

Author Small, David M.
Sanchez, Washington Y.
Gobe, Glenda C.
Title of chapter Intravital multiphoton imaging of the kidney: tubular structure and metabolism
Title of book Kidney research: experimental protocols
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2016
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-4939-3353-2_12
Open Access Status Not yet assessed
Year available 2016
Series Methods in molecular biology
Edition Second edition
ISBN 9781493933518
ISSN 1064-3745
Editor Tim D. Hewitson
Edward R. Smith
Stephen G. Holt
Volume number 1397
Chapter number 12
Start page 155
End page 172
Total pages 18
Total chapters 19
Collection year 2017
Language eng
Abstract/Summary Multiphoton microscopy (MPM) allows the visualization of dynamic pathophysiological events in real time in live animals. Intravital imaging can be applied to investigate novel mechanisms and treatments of different forms of kidney disease as well as improve our understanding of normal kidney physiology. Using rodent models, in conjunction with endogenous fluorescence and infused exogenous fluorescent dyes, measurement can be made of renal processes such as glomerular permeability, juxtaglomerular apparatus function, interactions of the tubulointerstitium, tubulovascular interactions, vascular flow rate, and the renin-angiotensin-aldosterone system. Subcellular processes including mitochondrial dynamics, reactive oxygen species production, cytosolic ion concentrations, and death processes of apoptosis and necrosis can also be seen and measured by MPM. The current methods chapter presents an overview of MPM with a focus on techniques for intravital kidney imaging and gives examples of instances where intravital MPM has been utilized to study renal pathophysiology. Suggestions are provided for MPM methods within the confines of intravital microscopy and selected kidney structure. MPM is undoubtedly a powerful new technique for application in experimental nephrology, and we believe it will continue to create new paradigms for understanding and treating kidney disease.
Keyword Fluorescence
Intravital imaging
Two-photon excitation
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status UQ

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