Efficient and durable vaccine against Intimin β of Diarrheagenic E. Coli induced by clay nanoparticles

Chen, Weiyu, Zhang, Bing, Mahony, Timothy, Gu, Wenyi, Rolfe, Barbara and Xu, Zhi Ping (2016) Efficient and durable vaccine against Intimin β of Diarrheagenic E. Coli induced by clay nanoparticles. Small, 12 12: 1627-1639. doi:10.1002/smll.201503359


Author Chen, Weiyu
Zhang, Bing
Mahony, Timothy
Gu, Wenyi
Rolfe, Barbara
Xu, Zhi Ping
Title Efficient and durable vaccine against Intimin β of Diarrheagenic E. Coli induced by clay nanoparticles
Formatted title
Efficient and durable vaccine against Intimin β of Diarrheagenic E. Coli induced by clay nanoparticles
Journal name Small   Check publisher's open access policy
ISSN 1613-6829
1613-6810
Publication date 2016-03-23
Sub-type Article (original research)
DOI 10.1002/smll.201503359
Open Access Status Not Open Access
Volume 12
Issue 12
Start page 1627
End page 1639
Total pages 13
Place of publication Weinheim, Germany
Publisher Wiley-VCH
Collection year 2017
Language eng
Formatted abstract
Improved strategies are urgently required to control infections with enterohemorrhagic Escherichia coli and enteropathogenic E. coli, two dominant zoonotic enteric pathogens responsible for a wide spectrum of illnesses as well as deaths of human being, with tremendous financial cost worldwide. The present study investigates the capacity of two clay nanoparticles (NPs) with opposite surface charges, namely synthetic layered double hydroxide (LDH) and hectorite (HEC) NPs as adjuvants to promote strong immune responses against the infections. Here both LDH and HEC NPs are showed to be able to carry an appreciable amount of Intimin β (1.1 and 4.4 mg per mg clay nanomaterials, respectively) and significantly facilitate antigen uptake by antigen-presenting cells. Remarkably, these clay NPs induce strong antibody and cell-mediated immune responses, which are much higher than that by the potent adjuvant, QuilA. Furthermore, these strong immune responses are well maintained for at least four months in the mouse model, during which there are no changes in histopathology of the animal organs. Collectively these data demonstrate the suitability of LDH and HEC NPs as useful adjuvants in new-generation vaccine formulations to control various infectious diseases.
Keyword Clay nanoparticles
Hectorite
Intimin β
Layered double hydroxides
Vaccine adjuvants
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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