Type B heterotrimeric G protein γ-subunit regulates Auxin and ABA signaling in tomato

Subramaniam, Gayathery, Trusov, Yuri, Lopez-Encina, Carlos, Hayashi, Satomi, Batley, Jacqueline and Botella, Jose Ramon (2016) Type B heterotrimeric G protein γ-subunit regulates Auxin and ABA signaling in tomato. Plant Physiology, 170 2: 1117-1134. doi:10.1104/pp.15.01675


Author Subramaniam, Gayathery
Trusov, Yuri
Lopez-Encina, Carlos
Hayashi, Satomi
Batley, Jacqueline
Botella, Jose Ramon
Title Type B heterotrimeric G protein γ-subunit regulates Auxin and ABA signaling in tomato
Journal name Plant Physiology   Check publisher's open access policy
ISSN 1532-2548
0032-0889
Publication date 2016-02-01
Year available 2015
Sub-type Article (original research)
DOI 10.1104/pp.15.01675
Open Access Status DOI
Volume 170
Issue 2
Start page 1117
End page 1134
Total pages 18
Place of publication Rockville, MD United States
Publisher American Society of Plant Biologists
Collection year 2016
Language eng
Formatted abstract
Heterotrimeric G proteins composed of α, β, and γ subunits are central signal transducers mediating the cellular response to multiple stimuli in most eukaryotes. Gγ subunits provide proper cellular localization and functional specificity to the heterotrimer complex. Plant Gγ subunits, divided into three structurally distinct types, are more diverse than their animal counterparts. Type B Gγ subunits, lacking a carboxyl-terminal isoprenylation motif, are found only in flowering plants. We present the functional characterization of type B Gγ subunit (SlGGB1) in tomato (Solanum lycopersicum). We show that SlGGB1 is the most abundant Gγ subunit in tomato and strongly interacts with the Gβ subunit. Importantly, the green fluorescent protein-SlGGB1 fusion protein as well as the carboxyl-terminal yellow fluorescent protein-SlGGB1/amino-terminal yellow fluorescent protein-Gβ heterodimer were localized in the plasma membrane, nucleus, and cytoplasm. RNA interference-mediated silencing of SlGGB1 resulted in smaller seeds, higher number of lateral roots, and pointy fruits. The silenced lines were hypersensitive to exogenous auxin, while levels of endogenous auxins were lower or similar to those of the wild type. SlGGB1-silenced plants also showed strong hyposensitivity to abscisic acid (ABA) during seed germination but not in other related assays. Transcriptome analysis of the transgenic seeds revealed abnormal expression of genes involved in ABA sensing, signaling, and response. We conclude that the type B Gγ subunit SlGGB1 mediates auxin and ABA signaling in tomato.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2016 Collection
 
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