The Fdb3 transcription factor of the Fusarium Detoxification of Benzoxazolinone gene cluster is required for MBOA but not BOA degradation in Fusarium pseudograminearum

Kettle, Andrew J., Carere, Jason, Batley, Jacqueline, Manners, John M., Kazan, Kemal and Gardiner, Donald M. (2016) The Fdb3 transcription factor of the Fusarium Detoxification of Benzoxazolinone gene cluster is required for MBOA but not BOA degradation in Fusarium pseudograminearum. Fungal Genetics and Biology, 88 44-53. doi:10.1016/j.fgb.2016.01.015


Author Kettle, Andrew J.
Carere, Jason
Batley, Jacqueline
Manners, John M.
Kazan, Kemal
Gardiner, Donald M.
Title The Fdb3 transcription factor of the Fusarium Detoxification of Benzoxazolinone gene cluster is required for MBOA but not BOA degradation in Fusarium pseudograminearum
Formatted title
The Fdb3 transcription factor of the Fusarium Detoxification of Benzoxazolinone gene cluster is required for MBOA but not BOA degradation in Fusarium pseudograminearum
Journal name Fungal Genetics and Biology   Check publisher's open access policy
ISSN 1096-0937
1087-1845
Publication date 2016-03-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.fgb.2016.01.015
Open Access Status Not Open Access
Volume 88
Start page 44
End page 53
Total pages 10
Place of publication Maryland Heights, MO United States
Publisher Academic Press
Collection year 2017
Language eng
Formatted abstract
A number of cereals produce the benzoxazolinone class of phytoalexins. Fusarium species pathogenic towards these hosts can typically degrade these compounds via an aminophenol intermediate, and the ability to do so is encoded by a group of genes found in the Fusarium Detoxification of Benzoxazolinone (FDB) cluster. A zinc finger transcription factor encoded by one of the FDB cluster genes (FDB3) has been proposed to regulate the expression of other genes in the cluster and hence is potentially involved in benzoxazolinone degradation. Herein we show that Fdb3 is essential for the ability of Fusarium pseudograminearum to efficiently detoxify the predominant wheat benzoxazolinone, 6-methoxy-benzoxazolin-2-one (MBOA), but not benzoxazoline-2-one (BOA). Furthermore, additional genes thought to be part of the FDB gene cluster, based upon transcriptional response to benzoxazolinones, are regulated by Fdb3. However, deletion mutants for these latter genes remain capable of benzoxazolinone degradation, suggesting that they are not essential for this process.
Keyword Phytoanticipin
Phytoalexin
Fusarium graminearum
Fusarium verticillioides
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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