Hematology of dugongs (Dugong dugon) in southern Queensland

Woolford, Lucy, Wong, Arthur, Sneath, Helen L., Long, Trevor, Boyd, Susan P. and Lanyon, Janet M. (2015) Hematology of dugongs (Dugong dugon) in southern Queensland. Veterinary Clinical Pathology, 44 4: 530-541. doi:10.1111/vcp.12305


Author Woolford, Lucy
Wong, Arthur
Sneath, Helen L.
Long, Trevor
Boyd, Susan P.
Lanyon, Janet M.
Title Hematology of dugongs (Dugong dugon) in southern Queensland
Formatted title
Hematology of dugongs (Dugong dugon) in southern Queensland
Journal name Veterinary Clinical Pathology   Check publisher's open access policy
ISSN 0275-6382
1939-165X
Publication date 2015-12
Year available 2015
Sub-type Article (original research)
DOI 10.1111/vcp.12305
Open Access Status Not Open Access
Volume 44
Issue 4
Start page 530
End page 541
Total pages 12
Place of publication Hoboken, NJ United States
Publisher Wiley-Blackwell Publishing
Collection year 2016
Language eng
Formatted abstract
Background
Little is known of the hematology of the dugong (Dugong dugon), a secretive and endangered coastal marine mammal.

Objectives
This paper reports hematologic reference intervals (RI) for dugongs and characterizes morphologic, cytochemical, and ultrastructural features of dugong leukocytes.

Methods
Blood was collected from live, apparently healthy dugongs and analyzed using Cell-Dyn 3700 or Sysmex XT-2000iV hematology analyzers. Blood films were subjected to a series of cytochemical stains, and leukocyte structure was examined using transmission electron microscopy.

Results
Reference intervals were established for 14 hematologic variables, total solids, and fibrinogen for 92 dugongs. Significant differences in some variables were found for animal size class, sex, and pregnancy status, and between analyzers. Subadults had higher leukocyte and lymphocyte counts than adults. Males had higher total solids and fibrinogen than females. Pregnant females had higher HCT, MCV, and circulating nucleated RBC, and lower platelet counts than nonpregnant females. Lymphocytes were usually the predominant circulating leukocyte. Heterophil cytoplasmic granules were abundant, fine, round to ovoid, and intensely eosinophilic, and round to ovoid or rod-shaped, and variably electron dense in electron microscopy. Eosinophils contained larger round eosinophilic to orange cytoplasmic granules, which ultrastructurally were bicompartmental with a round eccentric electron-dense core. Cytochemical staining of dugong heterophils suggests biochemical similarity to those of manatees and elephants, and for eosinophils, similarity to those of elephants, ruminants, and equids.

Conclusions
Generation of hematologic RI and characterization of leukocyte morphology improves evaluation of dugong health across this population and serves as a reference for other populations outside southern Queensland.
Keyword Blood
Cytochemistry
Morphology
Sirenia
Ultrastructure
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biological Sciences Publications
 
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