Immunocytochemical localization of angiotensinogen in the rat brain

Thomas, WG and Sernia, C (1988) Immunocytochemical localization of angiotensinogen in the rat brain. Neuroscience, 25 1: 319-341. doi:10.1016/0306-4522(88)90029-2

Author Thomas, WG
Sernia, C
Title Immunocytochemical localization of angiotensinogen in the rat brain
Journal name Neuroscience   Check publisher's open access policy
ISSN 0306-4522
Publication date 1988
Sub-type Article (original research)
DOI 10.1016/0306-4522(88)90029-2
Volume 25
Issue 1
Start page 319
End page 341
Total pages 23
Language eng
Subject 2800 Neuroscience
Abstract The distribution of angiotensinogen-like immunoreactivity in the rat brain was investigated using specific antisera against pure rat plasma angiotensinogen in conjunction with the sensitive streptavidin biotin peroxidase method. Angiotensinogen antisera were shown by radioimmunoassay and Western blotting to recognize angiotensinogen from both rat plasma and cerebrospinal fluid, and to cross-react with des-Al-angiotensinogen (100%) but not with angiotensin I and II. tetradecapeptide. luteinizing hormone-releasing hormone, rat albumin and angiotensinogen from eight other species. Angiotensinogen-like immunoreactivity was detected throughout the rat brain in both neuroglia and neurons. The highest concentration of neuroglial angiotensinogen-like immunoreactivity was in the hypothalamus and preoptic areas, with moderate to heavy concentrations in the mesencephalon and myelencephalon. The cerebellum demonstrated neuroglial staining in the granular layer and fibre tracts. Very little neuroglial staining was noted in the cerebral cortex or olfactory bulbs. Neuronal immunostaining was observed throughout the globus pallidus and the caudate putamen, in various parts of the thalamus and the supraoptic nucleus of the hypothalamus. In the midhrain moderate immunostaining was observed in periaquaductal central gray, the deep mesencephalic nucleus, the inferior colliculus and in scattered cells in the anterior mesencephalon. In the medulla, neuronal staining was localized to the vestibular nuclei and to other cell bodies mainly in the dorsolateral regions. In the cerebellum, staining was noted mainly in the deeper cerebellar nuclei and in the Purkinje cells. Immunostaining in the cerebral cortex was localized to the cingulate cortex and the primary olfactory cortex. Light staining was present in the endopiriform cortex and in scattered neurons adjacent to the external capsule. In the olfactory bulbs light neuronal staining was mainly associated with the mitral cell layer. The widespread distribution of angiotensinogen-like immunoreactivity supports the view that it is synthesized in the central nervous system and forms part of a brain renin angiotensin system. In addition, its presence at sites other than those normally associated with the control of blood pressure and fluid and electrolyte homeostasis suggests that its involvement may not be limited to these regulatory functions.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: ResearcherID Downloads
Scopus Import
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 83 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 76 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 12 Feb 2016, 19:15:09 EST by System User