Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags

Wang, Yuling, Wee, Eugene J. H. and Trau, Matt (2016) Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags. Chemical Communications, 52 17: 3560-3563. doi:10.1039/C6CC00547K


Author Wang, Yuling
Wee, Eugene J. H.
Trau, Matt
Title Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags
Journal name Chemical Communications   Check publisher's open access policy
ISSN 1364-548X
1359-7345
Publication date 2016-01-25
Sub-type Article (original research)
DOI 10.1039/C6CC00547K
Open Access Status Not Open Access
Volume 52
Issue 17
Start page 3560
End page 3563
Total pages 4
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Collection year 2017
Language eng
Formatted abstract
Sensitive and accurate total genomic DNA methylation analysis was demonstrated by surface-enhanced Raman scattering (SERS) via embedded internal SERS nanotags. The assay was sensitive to 0.2 ng input DNA while differentiating as low as 6.25% changes in DNA methylation. The method could also successfully differentiate cells before and after de-methylating drug-treatment and between tumor and normal biopsies.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Fri, 12 Feb 2016, 10:40:22 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences