Gestational diabetes mellitus is associated with changes in the concentration and bioactivity of placenta-derived exosomes in maternal circulation across gestation

Salomon, Carlos, Scholz-Romero, Katherin, Sarker, Suchismita, Sweeney, Emma, Kobayashi, Miharu, Correa, Paula, Longo, Sherri, Duncombe, Gregory, Mitchell, Murray D., Rice, Gregory E. and Illanes, Sebastian E. (2016) Gestational diabetes mellitus is associated with changes in the concentration and bioactivity of placenta-derived exosomes in maternal circulation across gestation. Diabetes, 65 3: 598-609. doi:10.2337/db15-0966


Author Salomon, Carlos
Scholz-Romero, Katherin
Sarker, Suchismita
Sweeney, Emma
Kobayashi, Miharu
Correa, Paula
Longo, Sherri
Duncombe, Gregory
Mitchell, Murray D.
Rice, Gregory E.
Illanes, Sebastian E.
Title Gestational diabetes mellitus is associated with changes in the concentration and bioactivity of placenta-derived exosomes in maternal circulation across gestation
Journal name Diabetes   Check publisher's open access policy
ISSN 0012-1797
1939-327X
Publication date 2016-03
Sub-type Article (original research)
DOI 10.2337/db15-0966
Open Access Status Not Open Access
Volume 65
Issue 3
Start page 598
End page 609
Total pages 12
Place of publication Alexandria, VA, United States
Publisher American Diabetes Association
Collection year 2017
Language eng
Formatted abstract
Although there is significant interest in elucidating the role of placenta-derived exosomes (PdEs) during pregnancy, the exosomal profile in pregnancies complicated by gestational diabetes mellitus (GDM) remains to be established. The aim of this study was to compare the gestational-age profile of PdEs in maternal plasma of GDM with normal pregnancies and to determine the effect of exosomes on cytokine release from human umbilical vein endothelial cells. A prospective cohort of patients was sampled at three time points during pregnancy for each patient (i.e., 11–14, 22–24, and 32–36 weeks' gestation). A retrospective stratified study design was used to quantify exosomes present in maternal plasma of normal (n = 13) and GDM (n = 7) pregnancies. Gestational age and pregnancy status were identified as significant factors contributing to variation in plasma exosome concentration (ANOVA, P < 0.05). Post hoc analyses established that PdE concentration increased during gestation in both normal and GDM pregnancies; however, the increase was significantly greater in GDM (∼2.2-fold, ∼1.5-fold, and ∼1.8-fold greater at each gestational age compared with normal pregnancies). Exosomes isolated from GDM pregnancies significantly increased the release of proinflammatory cytokines from endothelial cells. Although the role of exosomes during GDM remains to be fully elucidated, exosome profiles may be of diagnostic utility for screening asymptomatic populations.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
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Created: Thu, 11 Feb 2016, 13:57:21 EST by Roheen Gill on behalf of UQ Centre for Clinical Research