Cloning and sequence analysis of RNA-2 of a mechanically transmitted UK isolate of barley mild mosaic bymovirus (BaMMV)

Jacobi, V, Peerenboom, E, Schenk, PM, Antoniw, JF, Steinbiss, HH and Adams, MJ (1995) Cloning and sequence analysis of RNA-2 of a mechanically transmitted UK isolate of barley mild mosaic bymovirus (BaMMV). Virus Research, 37 2: 99-111. doi:10.1016/0168-1702(95)00024-K


Author Jacobi, V
Peerenboom, E
Schenk, PM
Antoniw, JF
Steinbiss, HH
Adams, MJ
Title Cloning and sequence analysis of RNA-2 of a mechanically transmitted UK isolate of barley mild mosaic bymovirus (BaMMV)
Journal name Virus Research   Check publisher's open access policy
ISSN 0168-1702
Publication date 1995
Sub-type Article (original research)
DOI 10.1016/0168-1702(95)00024-K
Volume 37
Issue 2
Start page 99
End page 111
Total pages 13
Language eng
Subject 1306 Cancer Research
2406 Virology
2725 Infectious Diseases
1312 Molecular Biology
Abstract A mutant of the 'Streatley' isolate of barley mild mosaic bymovirus was selected from the original field isolate by repeated mechanical incoulation. Unlike the wild-type barley mild mosaic virus, which is transmitted by the soilborne fungus Polymyxa graminis, the mutant could not be transmitted by this vector. RNA-2 of the mutant virus was shorter than that of the wild-type virus suggesting that a deletion of part of the genome segment had occurred. The nucleotide sequence of the mutant RNA-2 was determined and revealed a high degree of homology with the RNA-2 of a German BaMMV field isolate. The deletion comprises 1092 nucleotides and is located in the 3′-terminal part of the coding region. The 34-kDa truncated form of the C-terminal protein is less than half the size of the corresponding protein of full-length BaMMV RNA-2. Taken together, the sequence data and results of biological experiments suggest an essential role of the C-terminal protein for fungus transmission of BaMMV.
Keyword Barley mild mosaic virus (BaMMV)
Bymoviruses
Fungus transmission
Nucleotide sequence
RNA-2 deletion
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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