Incorporation of fluorescein conjugated function-spacer-lipid constructs into the red blood cell membrane facilitates detection of labeled cells for the duration of ex-vivo storage

Ki, Katrina K., Flower, Robert L., Faddy, Helen M. and Dean, Melinda M. (2016) Incorporation of fluorescein conjugated function-spacer-lipid constructs into the red blood cell membrane facilitates detection of labeled cells for the duration of ex-vivo storage. Journal of Immunological Methods, 429 66-70. doi:10.1016/j.jim.2016.01.003


Author Ki, Katrina K.
Flower, Robert L.
Faddy, Helen M.
Dean, Melinda M.
Title Incorporation of fluorescein conjugated function-spacer-lipid constructs into the red blood cell membrane facilitates detection of labeled cells for the duration of ex-vivo storage
Journal name Journal of Immunological Methods   Check publisher's open access policy
ISSN 1872-7905
0022-1759
Publication date 2016-02
Sub-type Article (original research)
DOI 10.1016/j.jim.2016.01.003
Open Access Status Not Open Access
Volume 429
Start page 66
End page 70
Total pages 5
Place of publication Amsterdam, NX, Netherlands
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
The contribution of ex-vivo storage duration of packed red blood cells (PRBC) to patient outcomes and transfusion-related immunomodulation (TRIM) remains a broadly debated area in transfusion medicine. Kode™ Technology with fluorescein conjugated function-spacer-lipid (FSL-FLRO4) constructs is a tool that can aid in-vitro visualization and tracking of red blood cells (RBC) during routine storage. FSL-FLRO4 is incorporated into the RBC membrane without altering cell function. In this study, we explore the suitability of this technology to label clinical grade PRBC and to determine if the label would be retained during ex-vivo storage. Firstly, to confirm feasibility and assess the limit of detection of FSL-FLRO4 on PRBC at date of expiry (42 days post-collection), we tracked the binding of FSL-FLRO4 on PRBC at weekly intervals during routine storage. Over the time course, all cells remained labelled with FSL-FLRO4, although a decrease in the intensity of labelling was observed (P < 0.0001). We then further investigated differences in FSL-FLRO4 labelling during RBC storage by labelling separated light-young and dense-old RBC from the same PRBC unit. There were no differences in the capacity of FSL-FLRO4 to label these different RBC subsets. Together, these data demonstrate that FSL-FLRO4 is a suitable reagent for labelling PRBC at any point during routine storage. This technology will facilitate the development of immunoassays and transfusion models focused on addressing the mechanisms involved in TRIM.
Keyword Ex-vivo storage
Function-spacer-lipid
PRBC
RBC labelling
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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