Phenotypic, functional, and plasticity features of classical and alternatively activated human macrophages

Tarique, Abdullah A., Logan, Jayden, Thomas, Emma, Holt, Patrick G., Sly, Peter D. and Fantino, Emmanuelle (2015) Phenotypic, functional, and plasticity features of classical and alternatively activated human macrophages. American Journal of Respiratory Cell and Molecular Biology, 53 5: 676-688. doi:10.1165/rcmb.2015-0012OC


Author Tarique, Abdullah A.
Logan, Jayden
Thomas, Emma
Holt, Patrick G.
Sly, Peter D.
Fantino, Emmanuelle
Title Phenotypic, functional, and plasticity features of classical and alternatively activated human macrophages
Journal name American Journal of Respiratory Cell and Molecular Biology   Check publisher's open access policy
ISSN 1044-1549
1535-4989
Publication date 2015-11
Year available 2015
Sub-type Article (original research)
DOI 10.1165/rcmb.2015-0012OC
Open Access Status Not Open Access
Volume 53
Issue 5
Start page 676
End page 688
Total pages 13
Place of publication New York, NY United States
Publisher American Thoracic Society
Collection year 2016
Language eng
Formatted abstract
Macrophages are dynamic cells that mature under the influence of signals from the local microenvironment into either classically (M1) or alternatively (M2) activated macrophages with specific functional and phenotypic properties. Although the phenotypic identification of M1 and M2 macrophages is well established in mice, this is less clear for human macrophages. In addition, the persistence and reversibility of polarized human phenotypes is not well established. Human peripheral blood monocytes were differentiated into uncommitted macrophages (M0) and then polarized to M1 and M2 phenotypes using LPS/IFN-γ and IL-4/IL-13, respectively. M1 and M2 were identified as CD64+CD80+ and CD11b+CD209+, respectively, by flow cytometry. Polarized M1 cells secreted IP-10, IFN-γ, IL-8, TNF-α, IL-1β, and RANTES, whereas M2 cells secreted IL-13, CCL17, and CCL18. Functionally, M2 cells were highly endocytic. In cytokine-deficient medium, the polarized macrophages reverted back to the M0 state within 12 days. If previously polarized macrophages were given the alternative polarizing stimulus after 6 days of resting in cytokine-deficient medium, a switch in polarization was seen (i.e., M1 macrophages switched to M2 and expressed CD11b+CD209+ and vice versa). In summary, we report phenotypic identification of human M1 and M2 macrophages, their functional characteristics, and their ability to be reprogrammed given the appropriate stimuli.
Keyword Classically activated macrophages (CAM/M1)
Alternatively activated macrophages (AAM/M2)
Phagocytosis/endocytosis
Phenotypic stability
Reprogramming of polarization
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
Child Health Research Centre Publications
 
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