Yeast three-hybrid system for the detection of protein-protein interactions

Maruta, Natsumi, Trusov, Yuri and Botella, Jose R. (2016). Yeast three-hybrid system for the detection of protein-protein interactions. In Jose R. Botella and Miguel A. Botella (Ed.), Plant signal transduction Second edition ed. (pp. 145-154) New York, NY, United States: Humana Press. doi:10.1007/978-1-4939-3115-6_12


Author Maruta, Natsumi
Trusov, Yuri
Botella, Jose R.
Title of chapter Yeast three-hybrid system for the detection of protein-protein interactions
Title of book Plant signal transduction
Place of Publication New York, NY, United States
Publisher Humana Press
Publication Year 2016
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-4939-3115-6_12
Open Access Status Not yet assessed
Year available 2016
Series Methods in Molecular Biology
Edition Second edition
ISBN 9781493931149
9781493931156
ISSN 1064-3745
1940-6029
Editor Jose R. Botella
Miguel A. Botella
Volume number 1363
Chapter number 12
Start page 145
End page 154
Total pages 10
Total chapters 19
Collection year 2017
Language eng
Abstract/Summary Protein-protein interaction studies provide useful insights into biological processes taking place within the living cell. A number of techniques are available to unravel large structural protein complexes, functional protein modules, and temporary protein associations occurring during signal transduction. The choice of method depends on the nature of the proteins and the interaction being studied. Here we present an optimized and simplified yeast three-hybrid method for analysis of protein interactions involving three components.
Keyword Protein complexes
Protein interaction
Signal transduction
Signaling cascades
Yeast three hybrid
Yeast two hybrid
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Book Chapter
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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