4,4′-dithiobisdipicolinic acid: a small and convenient lanthanide binding tag for protein NMR spectroscopy

Jia, Xinying, Maleckis, Ansis, Huber, Thomas and Otting, Gottfried (2011) 4,4′-dithiobisdipicolinic acid: a small and convenient lanthanide binding tag for protein NMR spectroscopy. Chemistry - A European Journal, 17 24: 6830-6836. doi:10.1002/chem.201003573


Author Jia, Xinying
Maleckis, Ansis
Huber, Thomas
Otting, Gottfried
Title 4,4′-dithiobisdipicolinic acid: a small and convenient lanthanide binding tag for protein NMR spectroscopy
Journal name Chemistry - A European Journal   Check publisher's open access policy
ISSN 0947-6539
1521-3765
Publication date 2011-06-06
Year available 2011
Sub-type Article (original research)
DOI 10.1002/chem.201003573
Open Access Status Not yet assessed
Volume 17
Issue 24
Start page 6830
End page 6836
Total pages 7
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Language eng
Abstract Pseudocontact shifts (PCS) from paramagnetic lanthanide ions present powerful long-range structure restraints for studies of proteins by nuclear magnetic resonance spectroscopy. To elicit PCSs, the lanthanide must be attached site-specifically to the target protein. In addition, it needs to be attached rigidly to avoid averaging of the PCSs due to mobility with respect to the protein and it must not interfere with the function of the protein. Here, we present a dipicolinic acid reagent that spontaneously forms a disulfide bond with thiol groups of accessible cysteine residues. A minimal number of rotatable bonds between the cysteine side chain and the tag helps to minimise mobility. Combined with the small size of the tag and quantitative tagging yields, these features make it a highly attractive tool for generating structure restraints by paramagnetic lanthanides. Copyright
Keyword Lanthanides
NMR spectroscopy
Protein labeling
Pseudocontact shifts
Thiol-reactive reagent
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 17 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sat, 06 Feb 2016, 02:30:45 EST by System User on behalf of Learning and Research Services (UQ Library)