Induction of interferon and cell death in response to cytosolic DNA in chicken macrophages

Vitak, Nazarii, Hume, David A., Chappell, Keith J., Sester, David P. and Stacey, Katryn J. (2016) Induction of interferon and cell death in response to cytosolic DNA in chicken macrophages. Developmental and Comparative Immunology, 59 145-152. doi:10.1016/j.dci.2016.01.023

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Author Vitak, Nazarii
Hume, David A.
Chappell, Keith J.
Sester, David P.
Stacey, Katryn J.
Title Induction of interferon and cell death in response to cytosolic DNA in chicken macrophages
Journal name Developmental and Comparative Immunology   Check publisher's open access policy
ISSN 0145-305X
Publication date 2016-06
Sub-type Article (original research)
DOI 10.1016/j.dci.2016.01.023
Open Access Status Not Open Access
Volume 59
Start page 145
End page 152
Total pages 26
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Collection year 2017
Language eng
Abstract Responses to cytosolic DNA can protect against both infectious organisms and the mutagenic effect of DNA integration. Recognition of invading DNA is likely to be fundamental to eukaryotic cellular life, but has been described only in mammals. Introduction of DNA into chicken macrophages induced type I interferon mRNA via a pathway conserved with mammals, requiring the receptor cGAS and the signalling protein STING. A second pathway of cytosolic DNA recognition in mammalian macrophages, initiated by absent in melanoma 2 (AIM2), results in rapid inflammasome-mediated pyroptotic cell death. AIM2 is restricted to mammals. Nevertheless, chicken macrophages underwent lytic cell death within 15 min of DNA transfection. The mouse AIM2-mediated response requires double stranded DNA, but chicken cell death was maintained with denatured DNA. This appears to be a novel form of rapid necrotic cell death, which we propose is an ancient response rendered redundant in mammalian macrophages by the appearance of the AIM2 inflammasome. The retention of these cytosolic DNA responses through evolution, with both conserved and non-conserved mechanisms, suggests a fundamental importance in cellular defence.
Keyword Cell death
Cytosolic DNA
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
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Created: Fri, 05 Feb 2016, 10:55:08 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences