Control of growth regulatory and differentiation-specific genes in human epidermal keratinocytes by interferon γ: antagonism by retinoic acid and transforming growth factor β1

Saunders, Nicholas A. and Jetten, Anton M. (1994) Control of growth regulatory and differentiation-specific genes in human epidermal keratinocytes by interferon γ: antagonism by retinoic acid and transforming growth factor β1. Journal of Biological Chemistry, 269 3: 2016-2022.

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Author Saunders, Nicholas A.
Jetten, Anton M.
Title Control of growth regulatory and differentiation-specific genes in human epidermal keratinocytes by interferon γ: antagonism by retinoic acid and transforming growth factor β1
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
1083-351X
Publication date 1994-01-21
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 269
Issue 3
Start page 2016
End page 2022
Total pages 7
Place of publication Bethesda, MD 20814 United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
Interferon γ (IFN-γ) is a potent inducer of squamous differentiation in normal human epidermal keratinocytes. This induction is characterized by a > or = 95% decrease in the mRNA level of two growth regulatory genes, cdc2 and E2F-1, and a 7-15-fold increase in the expression of two squamous cell-specific genes, transglutaminase type I and cornifin. In contrast to the decrease in cdc2 and E2F-1 expression, the increase in transglutaminase type I and cornifin mRNAs by IFN-γ occurs after a lagtime of more than 12 h. These results are consistent with the hypothesis that in normal human epidermal keratinocyte cells irreversible growth arrest precedes the expression of the squamous-differentiated phenotype. The action of IFN-γ on the expression of squamous cell-specific genes is antagonized by retinoic acid and transforming growth factor β1. Both factors are potent suppressors of the induction of transglutaminase type I and cornifin; however, they do not prevent the commitment to irreversible growth arrest. Several squamous cell carcinoma cell lines do not show a detectable decrease in cdc2 or increase in transglutaminase type I mRNA levels after IFN-γ treatment and appear to be altered in their control of squamous differentiation.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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