Differential expression of two calmodulin genes in response to physical and chemical stimuli

Botella, JR and Arteca, RN (1994) Differential expression of two calmodulin genes in response to physical and chemical stimuli. Plant Molecular Biology, 24 5: 757-766. doi:10.1007/BF00029857

Author Botella, JR
Arteca, RN
Title Differential expression of two calmodulin genes in response to physical and chemical stimuli
Journal name Plant Molecular Biology   Check publisher's open access policy
ISSN 0167-4412
Publication date 1994
Sub-type Article (original research)
DOI 10.1007/BF00029857
Volume 24
Issue 5
Start page 757
End page 766
Total pages 10
Publisher Kluwer Academic Publishers
Language eng
Subject 1303 Specialist Studies in Education
1300 Biochemistry, Genetics and Molecular Biology
Abstract Two different calmodulin (CaM) cDNAs (MBCaM-1 and MBCaM-2) were isolated from a vigna radiata λgt 11 library by screening with a heterologous Arabidopsis cDNA probe (TCH-1). Both cDNAs are 85% homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by MBCaM-1 and MBCaM-2 are identical except for two conservative substitutions at positions 7 and 10. Southern analysis revealed that both cDNAs are encoded by different genes. Expression studies revealed different patterns of expression of both genes. MBCaM-1 mRNA exhibited a dramatic transient increase in response to touch, while MBCaM-2 expression showed a steady but small increase as compared to MBCaM-1. When plants were grown in complete darkness MBCaM-1 was undetectable and MBCaM-2 exhibited very low levels of expression. One hour after exposure of etiolated seedlings to light MBCaM-1 showed no change, while MBCaM-2 expression was increased. After a 6 h exposure to light there was an induction of both MBCaM-1 and MBCaM-2; however, the magnitude of this increase was much greater for MBCaM-2. When plants were grown under a 16 h light/8 h dark cycle the mRNA levels for MBCaM-1 were lower during the light period and increased during the beginning of the night cycle, while MBCaM-2 showed no change. Plants treated with indole-3-acetic acid had a peak in MBCaM-1 expression 6 h after treatment initiation with a slight decline 3 h after the peak, while MBCaM-2 showed a steady but small increase over time as compared to MBCaM-1. When plants were subjected to salt stress they showed an increase in MBCaM-1 expression 2 h after treatment initiation reaching a maximum after 4 h with no further increase after 6 h, while MBCaM-2 remained unchanged over the time course.
Keyword calcium
indole-3-acetic acid
salt stress
signal transduction
Vigna radiata
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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