Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein

Koenig, Andrew L., Baltrunaite, Kristina, Bower, Neil I., Rossi, Andrea, Stainier, Didier Y. R., Hogan, Benjamin M. and Sumanas, Saulius (2016) Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein. Developmental Biology, 411 1: 115-127. doi:10.1016/j.ydbio.2016.01.002


Author Koenig, Andrew L.
Baltrunaite, Kristina
Bower, Neil I.
Rossi, Andrea
Stainier, Didier Y. R.
Hogan, Benjamin M.
Sumanas, Saulius
Title Vegfa signaling promotes zebrafish intestinal vasculature development through endothelial cell migration from the posterior cardinal vein
Journal name Developmental Biology   Check publisher's open access policy
ISSN 1095-564X
0012-1606
Publication date 2016-03-01
Sub-type Article (original research)
DOI 10.1016/j.ydbio.2016.01.002
Open Access Status Not Open Access
Volume 411
Issue 1
Start page 115
End page 127
Total pages 13
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2017
Language eng
Abstract The mechanisms underlying organ vascularization are not well understood. The zebrafish intestinal vasculature forms early, is easily imaged using transgenic lines and in-situ hybridization, and develops in a stereotypical pattern thus making it an excellent model for investigating mechanisms of organ specific vascularization. Here, we demonstrate that the sub-intestinal vein (SIV) and supra-intestinal artery (SIA) form by a novel mechanism from angioblasts that migrate out of the posterior cardinal vein and coalesce to form the intestinal vasculature in an anterior to posterior wave with the SIA forming after the SIV. We show that vascular endothelial growth factor aa (vegfaa) is expressed in the endoderm at the site where intestinal vessels form and therefore likely provides a guidance signal. Vegfa/Vegfr2 signaling is required for early intestinal vasculature development with mutation in vegfaa or loss of Vegfr2 homologs causing nearly complete inhibition of the formation of the intestinal vasculature. Vegfc and Vegfr3 function, however, are dispensable for intestinal vascularization. Interestingly, ubiquitous overexpression of Vegfc resulted in an overgrowth of the SIV, suggesting that Vegfc is sufficient to induce SIV development. These results argue that Vegfa signaling directs endothelial cells to migrate out of existing vasculature and coalesce to form the intestinal vessels. It is likely that a similar mechanism is utilized during vascularization of other organs.
Keyword Intestinal
Organ
Vascular endothelial
Vegf
Zebrafish
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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