Immunohistochemical expression of SOX9 protein in immature, mature, and neoplastic canine Sertoli cells

Banco, Barbara, Palmieri, Chiara, Sironi, Giuseppe, Fantinato, Eleonora, Veronesi, Maria C., Groppetti, Debora, Giudice, Chiara, Martignoni, Benedetta and Grieco, Valeria (2015) Immunohistochemical expression of SOX9 protein in immature, mature, and neoplastic canine Sertoli cells. Theriogenology, 85 7: 1408-1414. doi:10.1016/j.theriogenology.2015.12.024

Author Banco, Barbara
Palmieri, Chiara
Sironi, Giuseppe
Fantinato, Eleonora
Veronesi, Maria C.
Groppetti, Debora
Giudice, Chiara
Martignoni, Benedetta
Grieco, Valeria
Title Immunohistochemical expression of SOX9 protein in immature, mature, and neoplastic canine Sertoli cells
Journal name Theriogenology   Check publisher's open access policy
ISSN 0093-691X
Publication date 2015-07-30
Sub-type Article (original research)
DOI 10.1016/j.theriogenology.2015.12.024
Open Access Status Not Open Access
Volume 85
Issue 7
Start page 1408
End page 1414
Total pages 8
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2016
Language eng
Abstract Sex-determining region Y box9 gene (SOX9) protein plays a pivotal role in male sexual development. It regulates the transcription of the anti-Müllerian hormone gene promoting development of testis cords, multiplication, and maturation of Sertoli cells (SCs) and maintenance of spermatogenesis in adult testis. The immunohistochemical expression of SOX9 in normal testes has been reported in humans, mice, and rats. The present study aimed to investigate the expression of SOX9 in canine SCs during testicular maturation and neoplastic transformation. Canine testicular samples derived from three fetuses, four newborns, four prepubertal puppies, five adult dogs, 31 Sertoli cell tumors (SCTs) (one metastasizing), and five Leydig cell tumors (LCTs) were selected from departmental archive and tested immunohistochemically with a polyclonal antibody against SOX9 (1:150). All SCs from fetal, neonatal, and adult testes had a strong and exclusively nuclear labeling for SOX9. In SCs from prepubertal testes, SOX9 staining was highly variable with one negative sample (one of four), two samples with exclusively nuclear staining (two of four), and one with both nuclear and cytoplasmic labeling (one of four). Leydig cells (LCs) and LCTs were always negative. All 31 SCTs were positive for SOX9. The expression of SOX9 was nuclear, nuclear and cytoplasmic, and exclusively cytoplasmic in 18 of 31, 11 of 31, and two of 31 SCTs, respectively. This first report on the immunohistochemical expression of SOX9 in canine testes reports that in normal SCs from fetal, neonatal, and adult testes SOX9 labeled the nucleus, as in humans and laboratory animals. The cytoplasmic labeling observed in one prepubertal pairs of testes and in 11 SCTs could reflect SC immaturity or dedifferentiation, paralleling results observed in rat testes. The expression of SOX9 in SCs and SCTs and its absence in LCs and LCTs suggests that SOX9 is a reliable diagnostic marker for both normal and neoplastic SCs.
Keyword Dog
Sertoli cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Veterinary Science Publications
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