Induction of MITF expression in human cholangiocarcinoma cells and hepatocellular carcinoma cells by cyclopamine, an inhibitor of the Hedgehog signaling

Samatiwat, Papavee, Takeda, Kazuhisa, Satarug, Soisungwan, Ohba, Koji, Kukongviriyapan, Veerapol and Shibahara, Shigeki (2016) Induction of MITF expression in human cholangiocarcinoma cells and hepatocellular carcinoma cells by cyclopamine, an inhibitor of the Hedgehog signaling. Biochemical and Biophysical Research Communications, 470 1: 144-149. doi:10.1016/j.bbrc.2016.01.010


Author Samatiwat, Papavee
Takeda, Kazuhisa
Satarug, Soisungwan
Ohba, Koji
Kukongviriyapan, Veerapol
Shibahara, Shigeki
Title Induction of MITF expression in human cholangiocarcinoma cells and hepatocellular carcinoma cells by cyclopamine, an inhibitor of the Hedgehog signaling
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
0006-291X
Publication date 2016-01-29
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2016.01.010
Open Access Status Not Open Access
Volume 470
Issue 1
Start page 144
End page 149
Total pages 6
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2017
Language eng
Abstract Microphthalmia-associated transcription factor (MITF) is a key regulator of differentiation of melanocytes and retinal pigment epithelial cells, but it also has functions in non-pigment cells. MITF consists of multiple isoforms, including widely expressed MITF-A and MITF-H. In the present study, we explored the potential role played by the Hedgehog signaling on MITF expression in two common types of primary liver cancer, using human cholangiocarcinoma cell lines, the KKU-100 and HuCCT1, along with the HepG2 human hepatocellular carcinoma cell line. Importantly, cholangiocarcinoma is characterized by the activated Hedgehog signaling. Here we show that MITF-A mRNA is predominantly expressed in all three human liver cancer cell lines examined. Moreover, cyclopamine, an inhibitor of the Hedgehog signalling, increased the expression levels of MITF proteins in HuCCT1 and HepG2 cells, but not in KKU-100 cells, suggesting that MITF expression may be down-regulated in some liver cancer cases.
Keyword Bile duct
Cholangiocyte
Cyclopamine
Hedgehog
Hepatocyte
MITF
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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