Western environment/lifestyle is associated with increased genome methylation and decreased gene expression in Chinese immigrants living in Australia

Zhang, Guicheng, Wang, Kui, Schultz, Ennee, Khoo, Siew-Kim, Zhang, Xiaopeng, Annamalay, Alicia, Laing, Ingrid A., Hales, Belinda J., Goldblatt, Jack and Le Souef, Peter N. (2016) Western environment/lifestyle is associated with increased genome methylation and decreased gene expression in Chinese immigrants living in Australia. Environmental and Molecular Mutagenesis, 57 1: 65-73. doi:10.1002/em.21989


Author Zhang, Guicheng
Wang, Kui
Schultz, Ennee
Khoo, Siew-Kim
Zhang, Xiaopeng
Annamalay, Alicia
Laing, Ingrid A.
Hales, Belinda J.
Goldblatt, Jack
Le Souef, Peter N.
Title Western environment/lifestyle is associated with increased genome methylation and decreased gene expression in Chinese immigrants living in Australia
Journal name Environmental and Molecular Mutagenesis   Check publisher's open access policy
ISSN 1098-2280
0893-6692
Publication date 2016-01
Year available 2015
Sub-type Article (original research)
DOI 10.1002/em.21989
Open Access Status Not Open Access
Volume 57
Issue 1
Start page 65
End page 73
Total pages 9
Place of publication Hoboken, NJ, United States
Publisher John Wiley and Sons
Collection year 2016
Language eng
Formatted abstract
Introduction: Several human diseases and conditions are disproportionally distributed in the world with a significant “Western-developed” vs. “Eastern-developing” gradient.
Methods: We compared genome-wide DNA methylation of peripheral blood mononuclear cells in 25 newly arrived Chinese immigrants living in a Western environment for less than 6 months (“Newly arrived”) with 23 Chinese immigrants living in the Western environment for more than two years (“Long-term”) with a mean of 8.7 years, using the Infinium HumanMethylation450 BeadChip. In a sub-group of both subject groups (n = 12 each) we also investigated genome-wide gene expression using a Human HT-12 v4 expression beadChip.
Results: There were 62.5% probes among the total number of 382,250 valid CpG sites with greater mean Beta (β) in “Long-term” than in “Newly arrived”. In the regions of CpG islands and gene promoters, compared with the CpG sites in all other regions, lower percentages of CpG sites with mean methylation levels in “Long-term” greater than “Newly arrived” were observed, but still >50%. The increase of methylation was associated with a general decrease of gene expression in Chinese immigrants living in the Western environment for a longer period of time. After adjusting for age, gender and other confounding factors the findings remained.
Conclusion: Chinese immigrants living in Australia for a longer period of time have increased overall genome methylation and decreased overall gene expression compared with newly arrived immigrants.
Keyword Developed countries
Developing countries
Gene expression
Methylation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Published online 16 December 2015

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mathematics and Physics
Official 2016 Collection
 
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