The first genome sequence of a metatherian herpesvirus: Macropodid herpesvirus 1

Vaz, Paola K., Mahony, Timothy J., Hartley, Carol A., Fowler, Elizabeth V., Ficorilli, Nino, Lee, Sang W., Gilkerson, James R., Browning, Glenn F. and Devlin, Joanne M. (2016) The first genome sequence of a metatherian herpesvirus: Macropodid herpesvirus 1. BMC Genomics, 17 1: . doi:10.1186/s12864-016-2390-2


Author Vaz, Paola K.
Mahony, Timothy J.
Hartley, Carol A.
Fowler, Elizabeth V.
Ficorilli, Nino
Lee, Sang W.
Gilkerson, James R.
Browning, Glenn F.
Devlin, Joanne M.
Title The first genome sequence of a metatherian herpesvirus: Macropodid herpesvirus 1
Journal name BMC Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2016-01-22
Sub-type Article (original research)
DOI 10.1186/s12864-016-2390-2
Open Access Status DOI
Volume 17
Issue 1
Total pages 9
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2017
Language eng
Formatted abstract
Background
While many placental herpesvirus genomes have been fully sequenced, the complete genome of a marsupial herpesvirus has not been described. Here we present the first genome sequence of a metatherian herpesvirus, Macropodid herpesvirus 1 (MaHV-1).

Results
The MaHV-1 viral genome was sequenced using an Illumina MiSeq sequencer, de novo assembly was performed and the genome was annotated. The MaHV-1 genome was 140 kbp in length and clustered phylogenetically with the primate simplexviruses, sharing 67 % nucleotide sequence identity with Human herpesviruses 1 and 2. The MaHV-1 genome contained 66 predicted open reading frames (ORFs) homologous to those in other herpesvirus genomes, but lacked homologues of UL3, UL4, UL56 and glycoprotein J. This is the first alphaherpesvirus genome that has been found to lack the UL3 and UL4 homologues. We identified six novel ORFs and confirmed their transcription by RT-PCR.

Conclusions
This is the first genome sequence of a herpesvirus that infects metatherians, a taxonomically unique mammalian clade. Members of the Simplexvirus genus are remarkably conserved, so the absence of ORFs otherwise retained in eutherian and avian alphaherpesviruses contributes to our understanding of the Alphaherpesvirinae. Further study of metatherian herpesvirus genetics and pathogenesis provides a unique approach to understanding herpesvirus-mammalian interactions.
Keyword Genome
Herpes simplex
Herpesvirus
Kangaroo
Macropod
Marsupial
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Agriculture and Food Innovation
 
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