Rapid, automated online SPE-LC-QTRAP-MS/MS method for the simultaneous analysis of 14 phthalate metabolites and 5 bisphenol analogues in human urine

Heffernan, A. L., Thompson, K., Eaglesham, G., Vijayasarathy, S., Mueller, J. F., Sly, P. D. and Gomez-Ramos, M. J. (2016) Rapid, automated online SPE-LC-QTRAP-MS/MS method for the simultaneous analysis of 14 phthalate metabolites and 5 bisphenol analogues in human urine. Talanta, 151 224-233. doi:10.1016/j.talanta.2016.01.037


Author Heffernan, A. L.
Thompson, K.
Eaglesham, G.
Vijayasarathy, S.
Mueller, J. F.
Sly, P. D.
Gomez-Ramos, M. J.
Title Rapid, automated online SPE-LC-QTRAP-MS/MS method for the simultaneous analysis of 14 phthalate metabolites and 5 bisphenol analogues in human urine
Journal name Talanta   Check publisher's open access policy
ISSN 0039-9140
1873-3573
Publication date 2016-05-01
Sub-type Article (original research)
DOI 10.1016/j.talanta.2016.01.037
Open Access Status Not Open Access
Volume 151
Start page 224
End page 233
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Phthalates and bisphenol A (BPA) have received special attention in recent years due to their frequent use in consumer products and potential for adverse effects on human health. BPA is being replaced with a number of alternatives, including bisphenol S, bisphenol B, bisphenol F and bisphenol AF. These bisphenol analogues have similar potential for adverse health effects, but studies on human exposure are limited. Accurate measurement of multiple contaminants is important for estimating exposure. This paper describes a sensitive and automated method for the simultaneous determination of 14 phthalate metabolites, BPA and four bisphenol analogues in urine using online solid phase extraction coupled with high-performance liquid chromatography/tandem mass spectrometry using a hybrid triple-quadrupole linear ion trap mass spectrometer (LC-QTRAP-MS/MS), requiring very little sample volume (50 µL). Quantification was performed under selected reaction monitoring (SRM) mode with negative electrospray ionization. The use of SRM combined with an enhanced product ion scan within the same analysis was examined. Unequivocal identification was provided by the acquisition of three SRM transitions per compound and isotope dilution. The analytical performance of the method was evaluated in synthetic and human urine. Linearity of response over three orders of magnitude was demonstrated for all of the compounds (R2>0.99), with method detection limits of 0.01–0.5 ng/mL and limits of reporting of 0.07–3.1 ng/mL. Accuracy ranged from 93% to 113% and inter- and intra-day precision were <22%. Finally, the validated method has been successfully applied to a cohort of pregnant women to measure biomarker concentrations of phthalates and bisphenols, with median concentrations ranging from 0.3 ng/mL (bisphenol S) to 18.5 ng/mL (monoethyl phthalate).
Keyword Phthalates
Bisphenol A
Bisphenol analogues
Biomonitoring
On-line solid phase extraction
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Mon, 01 Feb 2016, 21:45:49 EST by Amy Heffernan on behalf of National Res Centre For Environmental Toxicology