Cytorhabdovirus P protein suppresses RISC-mediated cleavage and RNA silencing amplification in planta

Mann, Krin S., Johnson, Karyn N., Carroll, Bernard J. and Dietzgen, Ralf G. (2016) Cytorhabdovirus P protein suppresses RISC-mediated cleavage and RNA silencing amplification in planta. Virology, 490 27-40. doi:10.1016/j.virol.2016.01.003


Author Mann, Krin S.
Johnson, Karyn N.
Carroll, Bernard J.
Dietzgen, Ralf G.
Title Cytorhabdovirus P protein suppresses RISC-mediated cleavage and RNA silencing amplification in planta
Formatted title
Cytorhabdovirus P protein suppresses RISC-mediated cleavage and RNA silencing amplification in planta
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
1089-862X
Publication date 2016-03
Sub-type Article (original research)
DOI 10.1016/j.virol.2016.01.003
Open Access Status Not Open Access
Volume 490
Start page 27
End page 40
Total pages 14
Place of publication Waltham, MA, United States
Publisher Academic Press
Collection year 2017
Language eng
Formatted abstract
Plant viruses have evolved to undermine the RNA silencing pathway by expressing suppressor protein(s) that interfere with one or more key components of this antiviral defense. Here we show that the recently identified RNA silencing suppressor (RSS) of lettuce necrotic yellows virus (LNYV), phosphoprotein P, binds to RNA silencing machinery proteins AGO1, AGO2, AGO4, RDR6 and SGS3 in protein-protein interaction assays when transiently expressed. In planta, we demonstrate that LNYV P inhibits miRNA-guided AGO1 cleavage and translational repression, and RDR6/SGS3-dependent amplification of silencing. Analysis of LNYV P deletion mutants identified a C-terminal protein domain essential for both local RNA silencing suppression and interaction with AGO1, AGO2, AGO4, RDR6 and SGS3. In contrast to other viral RSS known to disrupt AGO activity, LNYV P sequence does not contain any recognizable GW/WG or F-box motifs. This suggests that LNYV P may represent a new class of AGO binding proteins.
Keyword Agroinfiltration
Argonaute proteins
Lettuce necrotic yellows cytorhabdovirus
Phosphoprotein
Protein–protein interactions
RNA silencing amplification
RNA silencing suppression
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Fri, 29 Jan 2016, 13:41:22 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences