Soluble arabinoxylan enhances large intestinal microbial health biomarkers in pigs fed a red meat-containing diet

Williams, Barbara A., Zhang, Dagong, Lisle, Allan T., Mikkelsen, Deirdre, McSweeney C. S., Kang, Seungha, Bryden, Wayne L. and Gidley, Michael J. (2016) Soluble arabinoxylan enhances large intestinal microbial health biomarkers in pigs fed a red meat-containing diet. Nutrition, 32 4: 491-497. doi:10.1016/j.nut.2015.10.008


Author Williams, Barbara A.
Zhang, Dagong
Lisle, Allan T.
Mikkelsen, Deirdre
McSweeney C. S.
Kang, Seungha
Bryden, Wayne L.
Gidley, Michael J.
Title Soluble arabinoxylan enhances large intestinal microbial health biomarkers in pigs fed a red meat-containing diet
Journal name Nutrition   Check publisher's open access policy
ISSN 1873-1244
0899-9007
Publication date 2016-04
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.nut.2015.10.008
Open Access Status Not Open Access
Volume 32
Issue 4
Start page 491
End page 497
Total pages 8
Place of publication New York, United States
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Objectives: The aim of this study was to investigate how moderately increased dietary red meat combined with a soluble fiber (wheat arabinoxylan [AX]) alters the large intestinal microbiota in terms of fermentative end products and microbial community profiles in pigs.

Methods: Four groups of 10 pigs were fed Western-type diets containing two amounts of red meat, with or without a solubilized wheat AX-rich fraction for 4 wk. After euthanasia, fermentative end products (short-chain fatty acids, ammonia) of digesta from four sections of large intestine were measured. Di-amino-pimelic acid was a measure of total microbial biomass, and bacterial profiles were determined using a phylogenetic microarray. A factorial model determined effects of AX and meat content.

Results: Arabinoxylan was highly fermentable in the cecum, as indicated by increased concentrations of short-chain fatty acids (particularly propionate). Protein fermentation end products were decreased, as indicated by the reduced ammonia and branched-chain ratio although this effect was less prominent distally. Microbial profiles in the distal large intestine differed in the presence of AX (including promotion of Faecalibacterium prausnitzii), consistent with an increase in carbohydrate versus protein fermentation. Increased di-amino-pimelic acid (P < 0.0001) suggested increased microbial biomass for animals fed AX.

Conclusions: 
Solubilized wheat AX has the potential to counteract the effects of dietary red meat by reducing protein fermentation and its resultant toxic end products such as ammonia, as well as leading to a positive shift in fermentation end products and microbial profiles in the large intestine.
Keyword Wheat arabinoxylan
Soluble dietary fiber
Microbial biomass
Protein fermentation
Pig model
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Queensland Alliance for Agriculture and Food Innovation
Official 2016 Collection
 
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