Germ cells influence cord formation and leydig cell gene expression during mouse testis development

Rios-Rojas, Clarissa, Spiller, Cassy, Bowles, Josephine and Koopman, Peter (2016) Germ cells influence cord formation and leydig cell gene expression during mouse testis development. Developmental Dynamics, 245 4: 433-444. doi:10.1002/dvdy.24371


Author Rios-Rojas, Clarissa
Spiller, Cassy
Bowles, Josephine
Koopman, Peter
Title Germ cells influence cord formation and leydig cell gene expression during mouse testis development
Journal name Developmental Dynamics   Check publisher's open access policy
ISSN 1097-0177
1058-8388
Publication date 2016
Sub-type Article (original research)
DOI 10.1002/dvdy.24371
Volume 245
Issue 4
Start page 433
End page 444
Total pages 12
Place of publication Hoboken, NJ, United States
Publisher John Wiley and Sons
Collection year 2017
Language eng
Subject 1309 Developmental Biology
Formatted abstract
Background: It is widely accepted that, during the development of testes in the mammalian embryo, male germ cells are influenced by signals from the surrounding somatic cells, but not vice versa, so that germ cells are dispensable for the formation of testes.

Results: We now demonstrate that development of the mouse fetal testis is compromised in the absence of germ cells. Using two- and three-dimensional imaging techniques, we reveal that We/We mutant testes devoid of germ cells have misshapen and poorly organized cords. We also found that mutant gonads have fewer Sertoli cells than normal and that the Leydig cells express key markers at higher than normal levels.

Conclusions: These observations point to the existence of germ cell-derived signals that directly or indirectly affect the Sertoli and Leydig cell populations, and provide a new paradigm for the organogenesis of the mammalian testes.
Keyword Morphogenesis
Organogenesis
Sex determination
Signaling
Testosterone
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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