Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease

Walker, Meegan A., Hoier, Birgitte, Walker, Philip J., Schulze, Karl, Bangsbo, Jens, Hellsten, Ylva and Askew, Christopher D. (2016) Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease. Atherosclerosis, 246 98-105. doi:10.1016/j.atherosclerosis.2015.12.029

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Author Walker, Meegan A.
Hoier, Birgitte
Walker, Philip J.
Schulze, Karl
Bangsbo, Jens
Hellsten, Ylva
Askew, Christopher D.
Title Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease
Journal name Atherosclerosis   Check publisher's open access policy
ISSN 1879-1484
Publication date 2016-03
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.atherosclerosis.2015.12.029
Open Access Status File (Author Post-print)
Volume 246
Start page 98
End page 105
Total pages 8
Place of publication Shannon, Clare, Ireland
Publisher Elsevier
Collection year 2016
Language eng
Formatted abstract
Background: Peripheral arterial disease (PAD) is characterised by impaired leg blood flow, which contributes to claudication and reduced exercise capacity. This study investigated to what extent vasoactive enzymes might contribute to altered blood flow in PAD (Fontaine stage II).
Methods: We compared femoral artery blood flow during reactive hyperaemia, leg-extension exercise and passive leg movement, and determined the level of vasoactive enzymes in skeletal muscle samples from the vastus lateralis in PAD (n = 10, 68.5 ± 6.5 years) and healthy controls (CON, n = 9, 62.1 ± 12.3 years). Leg blood flow was measured with Doppler ultrasound and muscle protein levels of phosphorylated endothelial nitric oxide synthase, NADPH oxidase, cyclooxygenase 1 and 2, thromboxane synthase, and prostacyclin synthase were determined.
Results: Leg blood flow during the initial 90 s of passive leg movement (242 ± 33 vs 441 ± 75 ml min−1, P = 0.03) and during reactive hyperaemia (423 ± 100 vs 1255 ± 175 ml min−1, P = 0.002) was lower in PAD than CON, whereas no significant difference was observed for leg blood flow during exercise (1490 ± 250 vs 1887 ± 349 ml min−1, P = 0.37). PAD had higher NADPH oxidase than CON (1.04 ± 0.19 vs 0.50 ± 0.06 AU, P = 0.02), with no differences for other enzymes. Leg blood flow during exercise was correlated with prostacyclin synthase (P = 0.001).
Conclusion: Elevated NADPH oxidase indicates that oxidative stress may be a primary cause of low nitric oxide availability and impaired blood flow in PAD.
Keyword Leg blood flow
NADPH oxidase
Peripheral arterial disease
Vasoactive enzymes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Published online 24 December 2015

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
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