Genomic comparison of two O111:H- Enterohemorrhagic Escherichia coli isolates from a historic hemolytic uremic syndrome outbreak in Australia

McAllister, Lauren J., Bent, Stephen J., Petty, Nicola K., Skippington, Elizabeth, Beatson, Scott A., Paton, Jmaes C. and Paton, Adrienne W. (2016) Genomic comparison of two O111:H- Enterohemorrhagic Escherichia coli isolates from a historic hemolytic uremic syndrome outbreak in Australia. Infection and Immunity, 84 3: 775-781. doi:10.1128/IAI.01229-15

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ377035_OA.pdf Full text (open access) application/pdf 1.38MB 0

Author McAllister, Lauren J.
Bent, Stephen J.
Petty, Nicola K.
Skippington, Elizabeth
Beatson, Scott A.
Paton, Jmaes C.
Paton, Adrienne W.
Title Genomic comparison of two O111:H- Enterohemorrhagic Escherichia coli isolates from a historic hemolytic uremic syndrome outbreak in Australia
Formatted title
Genomic comparison of two O111:H- Enterohemorrhagic Escherichia coli isolates from a historic hemolytic uremic syndrome outbreak in Australia
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
1070-6313
Publication date 2016-03
Sub-type Article (original research)
DOI 10.1128/IAI.01229-15
Open Access Status File (Publisher version)
Volume 84
Issue 3
Start page 775
End page 781
Total pages 7
Place of publication Washington DC, United States
Publisher American Society for Microbiology
Collection year 2017
Language eng
Formatted abstract
Enterohemorrhagic Escherichia coli (EHEC) is an important cause of diarrhea and hemolytic-uremic syndrome (HUS) worldwide. Australia's worst outbreak of HUS occurred in Adelaide in 1995 and was one of the first major HUS outbreaks attributed to a non-O157 Shiga-toxigenic E. coli (STEC) strain. Molecular analyses conducted at the time suggested that the outbreak was caused by an O111:H clone, with strains from later in the outbreak harboring an extra copy of the genes encoding the potent Shiga toxin 2 (Stx2). Two decades later, we have used next-generation sequencing to compare two isolates from early and late in this important outbreak. We analyzed genetic content, single-nucleotide polymorphisms (SNPs), and prophage insertion sites; for the latter, we demonstrate how paired-end sequence data can be leveraged to identify such insertion sites. The two strains are genetically identical except for six SNP differences and the presence of not one but two additional Stx2-converting prophages in the later isolate. Isolates from later in the outbreak were associated with higher levels of morbidity, suggesting that the presence of the additional Stx2-converting prophages is significant in terms of the virulence of this clone.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Fri, 15 Jan 2016, 13:34:31 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences