Enrichment and identification of glycoproteins in human saliva using lectin magnetic bead arrays

Caragata, Michael, Shah, Alok K., Schulz, Benjamin L., Hill, Michelle M. and Punyadeera, Chamindie (2016) Enrichment and identification of glycoproteins in human saliva using lectin magnetic bead arrays. Analytical Biochemistry, 497 76-82. doi:10.1016/j.ab.2015.11.024


Author Caragata, Michael
Shah, Alok K.
Schulz, Benjamin L.
Hill, Michelle M.
Punyadeera, Chamindie
Title Enrichment and identification of glycoproteins in human saliva using lectin magnetic bead arrays
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
1096-030
Publication date 2016
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.ab.2015.11.024
Open Access Status Not Open Access
Volume 497
Start page 76
End page 82
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2016
Language eng
Formatted abstract
Aberrant glycosylation of proteins is a hallmark of tumorigenesis and could provide diagnostic value in cancer detection. Human saliva is an ideal source of glycoproteins due to the relatively high proportion of glycosylated proteins in the salivary proteome. Moreover, saliva collection is noninvasive and technically straightforward, and the sample collection and storage is relatively easy. Although differential glycosylation of proteins can be indicative of disease states, identification of differential glycosylation from clinical samples is not trivial. To facilitate salivary glycoprotein biomarker discovery, we optimized a method for differential glycoprotein enrichment from human saliva based on lectin magnetic bead arrays (saLeMBA). Selected lectins from distinct reactivity groups were used in the saLeMBA platform to enrich salivary glycoproteins from healthy volunteer saliva. The technical reproducibility of saLeMBA was analyzed with liquid chromatographyetandem mass spectrometry (LCeMS/MS) to identify the glycosylated proteins enriched by each lectin. Our saLeMBA platform enabled robust glycoprotein enrichment in a glycoprotein- and lectin-specific manner consistent with known protein-specific glycan profiles. We demonstrated that saLeMBA is a reliable method to enrich and detect glycoproteins present in human saliva.
Keyword Biomarker discovery
Glycosylation
Lectins
Magnetic Beads
Saliva proteomics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
UQ Diamantina Institute Publications
 
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Created: Fri, 15 Jan 2016, 12:18:50 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences