Illustrating ultra-low-volume rheology on a conventional rheometer: Charting the development of hyaluronan during fermentation

Boehm, Michael W., Shewan, Heather M., Steen, Jennifer A. and Stokes, Jason R. (2015) Illustrating ultra-low-volume rheology on a conventional rheometer: Charting the development of hyaluronan during fermentation. Applied Rheology, 25 55609: 12-19. doi:10.3933/APPLRHEOL-25-55609


Author Boehm, Michael W.
Shewan, Heather M.
Steen, Jennifer A.
Stokes, Jason R.
Title Illustrating ultra-low-volume rheology on a conventional rheometer: Charting the development of hyaluronan during fermentation
Journal name Applied Rheology   Check publisher's open access policy
ISSN 1430-6395
1617-8106
Publication date 2015-08-04
Year available 2015
Sub-type Article (original research)
DOI 10.3933/APPLRHEOL-25-55609
Open Access Status Not yet assessed
Volume 25
Issue 55609
Start page 12
End page 19
Total pages 8
Place of publication Lappersdorf, Germany
Publisher Kerschensteiner Verlag GmbH
Collection year 2016
Language eng
Formatted abstract
We provide methodologies to characterise the rheology of ultra-low volumes of polymer solutions and biological fluids (10 - 100 μL) on a rotational rheometer. The technique utilises a parallel plate geometry with narrow gaps of 20 - 100 micrometers, which is an order of magnitude less than conventional methods. Despite the complications these gaps present, the use of appropriate protocols ensures reliable and accurate rheological characterisation of fluids, including shear-dependent viscosity, normal stresses and linear viscoelasticity. This rheological technique.s usefulness is further demonstrated by showing how the rheology of hyaluronan solutions evolve during fermentation. The intrinsic viscosity of the hyaluronan macromolecule is determined using less than 100 μL of solution extracted directly from the bioreactor, and this is used to provide a reasonable indicator of its molecular weight as it develops during the fermentation process. The ability to measure rheology of ultra-low volumes has applications in the characterisation of biological fluids and high value macromolecules, as well as generally in biotechnology and nanotechnology research fields.
Keyword Ultra-low-volume reheology
Narrow gap
Hyaluronan
Intrinsic viscosity
Molecular weight
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Thu, 07 Jan 2016, 15:03:18 EST by Heather Shewan on behalf of School of Chemical Engineering