Evaluating the potential impact of proton carriers on syntrophic propionate oxidation

Juste-Poinapen, Natacha M.S., Turner, Mark S., Rabaey, Korneel, Virdis, Bernardino and Batstone, Damien J. (2015) Evaluating the potential impact of proton carriers on syntrophic propionate oxidation. Scientific Reports, 5 18364: . doi:10.1038/srep18364


Author Juste-Poinapen, Natacha M.S.
Turner, Mark S.
Rabaey, Korneel
Virdis, Bernardino
Batstone, Damien J.
Title Evaluating the potential impact of proton carriers on syntrophic propionate oxidation
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2015-12-16
Year available 2015
Sub-type Article (original research)
DOI 10.1038/srep18364
Open Access Status DOI
Volume 5
Issue 18364
Total pages 9
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2016
Language eng
Formatted abstract
Anaerobic propionic acid degradation relies on interspecies electron transfer (IET) between propionate oxidisers and electron acceptor microorganisms, via either molecular hydrogen, formate or direct transfers. We evaluated the possibility of stimulating direct IET, hence enhancing propionate oxidation, by increasing availability of proton carriers to decrease solution resistance and reduce pH gradients. Phosphate was used as a proton carrying anion, and chloride as control ion together with potassium as counter ion. Propionic acid consumption in anaerobic granules was assessed in a square factorial design with ratios (1:0, 2:1, 1:1, 1:2 and 0:1) of total phosphate (TP) to Cl−, at 1X, 10X, and 30X native conductivity (1.5 mS.cm−1). Maximum specific uptake rate, half saturation, and time delay were estimated using model-based analysis. Community profiles were analysed by fluorescent in situ hybridisation and 16S rRNA gene pyrosequencing. The strongest performance was at balanced (1:1) ratios at 10X conductivity where presumptive propionate oxidisers namely Syntrophobacter and Candidatus Cloacamonas were more abundant. There was a shift from Methanobacteriales at high phosphate, to Methanosaeta at low TP:Cl ratios and low conductivity. A lack of response to TP, and low percentage of presumptive electroactive organisms suggested that DIET was not favoured under the current experimental conditions.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2016 Collection
Advanced Water Management Centre Publications
 
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