Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion

Henry, Claire, Llamosas, Estelle, Knipprath-Meszaros, Alexandra, Schoetzau, Andreas, Obermann, Ellen, Fuenfschilling, Maya, Caduff, Rosemarie, Fink, Daniel, Hacker, Neville, Ward, Robyn, Heinzelmann-Schwarz, Viola and Ford, Caroline (2015) Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion. Oncotarget, 6 37: 40310-40326. doi:10.18632/oncotarget.5643


Author Henry, Claire
Llamosas, Estelle
Knipprath-Meszaros, Alexandra
Schoetzau, Andreas
Obermann, Ellen
Fuenfschilling, Maya
Caduff, Rosemarie
Fink, Daniel
Hacker, Neville
Ward, Robyn
Heinzelmann-Schwarz, Viola
Ford, Caroline
Title Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion
Journal name Oncotarget   Check publisher's open access policy
ISSN 1949-2553
Publication date 2015-10-20
Year available 2015
Sub-type Article (original research)
DOI 10.18632/oncotarget.5643
Open Access Status DOI
Volume 6
Issue 37
Start page 40310
End page 40326
Total pages 17
Place of publication Albany, NY, United States
Publisher Impact Journals LLC
Collection year 2016
Language eng
Formatted abstract
AIM: In recent years, the Wnt signalling pathway has been implicated in epithelial ovarian cancer and its members have potential as diagnostic, prognostic and therapeutic targets. Here we investigated the role of two Wnt receptor tyrosine kinases (RTKs), ROR1 and ROR2, and their putative ligand, Wnt5a, in ovarian cancer.

METHODS: Immunohistochemistry for ROR2 was performed in a large patient cohort, including benign controls, borderline tumours and epithelial ovarian cancer. In addition, siRNA was used to silence ROR1, ROR2 and Wnt5a individually, and together, in two ovarian cancer cell lines, and the effects on cell proliferation, adhesion, migration and invasion were measured.

RESULTS: ROR2 expression is significantly increased in ovarian cancer patients compared to patients with benign disease. In vitro assays showed that silencing either receptor inhibits ovarian cancer cell migration and invasion, and concurrently silencing both receptors has an even stronger inhibitory effect on proliferation, migration and invasion.

CONCLUSIONS:
ROR2 expression is increased in epithelial ovarian cancer, and silencing ROR2 and its sister receptor ROR1 has a strong inhibitory effect on the ability of ovarian cancer cells to proliferate, migrate and invade through an extracellular matrix.
Keyword Epithelial ovarian cancer
Metastasis
ROR1
ROR2
Wnt signalling
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Office of the Vice-Chancellor
Official 2016 Collection
 
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