Characterisation and cross-amplification of 21 novel microsatellite loci for the dusky shark, Carcharhinus obscurus

Blower, D. C., Corley, S., Hereward, J. P., Riginos, C. R. and Ovenden, J. R. (2015) Characterisation and cross-amplification of 21 novel microsatellite loci for the dusky shark, Carcharhinus obscurus. Conservation Genetics Resources, 7 4: 909-912. doi:10.1007/s12686-015-0499-2


 
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Author Blower, D. C.
Corley, S.
Hereward, J. P.
Riginos, C. R.
Ovenden, J. R.
Title Characterisation and cross-amplification of 21 novel microsatellite loci for the dusky shark, Carcharhinus obscurus
Formatted title
Characterisation and cross-amplification of 21 novel microsatellite loci for the dusky shark, Carcharhinus obscurus
Journal name Conservation Genetics Resources   Check publisher's open access policy
ISSN 1877-7252
1877-7260
Publication date 2015-12
Year available 2015
Sub-type Article (original research)
DOI 10.1007/s12686-015-0499-2
Open Access Status Not Open Access
Volume 7
Issue 4
Start page 909
End page 912
Total pages 4
Place of publication Dordrecht, Netherlands
Publisher Springer
Collection year 2016
Language eng
Formatted abstract
The dusky shark Carcharhinus obscurus risks excessive fisheries exploitation worldwide due to its low productivity. Genetic monitoring is an effective way of resolving species stock structure, genetic diversity, and forensically identifying processed animals. Here we present the first C. obscurus species-specific microsatellite loci. Twenty-one di- to tetra-nucleotide loci with between 2 and 20 alleles per locus were developed. Observed heterozygosity ranged from 0.28 to 0.91 with only one locus slightly deviating from Hardy–Weinberg equilibrium. No significant evidence for null alleles or linkage disequilibrium was detected. These loci were cross-amplified in three related species. Seventeen, twelve, and eighteen loci exhibited polymorphism in sandbar shark Carcharhinus plumbeus, spinner shark Carcharhinus brevipinna, galapagos shark Carcharhinus galapagensis, respectively. Investigations into C. obscurus will benefit from these loci which possess attributes suitable for population-scale and individual-scale analyses. Additionally locus cross-amplification will facilitate research for these species with few existing microsatellites and similarly vulnerable life-histories.
Keyword Carcharhinus obscurus
Dusky shark
Microsatellite
454 Sequencing
Cross-amplification
Carcharhinus plumbeus
Carcharhinus brevipinna
Carcharhinus galapagensis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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