PKCε as a novel promoter of skeletal muscle differentiation and regeneration

Di Marcantonio, D., Galli, D., Carubbi, C., Gobbi, G., Queirolo, V., Martini, S., Merighi, S., Vaccarezza, M., Maffulli, N., Sykes, S. M., Vitale, M. and Mirandola, P. (2015) PKCε as a novel promoter of skeletal muscle differentiation and regeneration. Experimental Cell Research, 339 1: 10-19. doi:10.1016/j.yexcr.2015.09.017

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Author Di Marcantonio, D.
Galli, D.
Carubbi, C.
Gobbi, G.
Queirolo, V.
Martini, S.
Merighi, S.
Vaccarezza, M.
Maffulli, N.
Sykes, S. M.
Vitale, M.
Mirandola, P.
Title PKCε as a novel promoter of skeletal muscle differentiation and regeneration
Journal name Experimental Cell Research   Check publisher's open access policy
ISSN 1090-2422
0014-4827
Publication date 2015-11-15
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.yexcr.2015.09.017
Open Access Status File (Author Post-print)
Volume 339
Issue 1
Start page 10
End page 19
Total pages 10
Place of publication Waltham, MA, United States
Publisher Academic Press
Collection year 2016
Language eng
Formatted abstract
Introduction

Satellite cells are muscle resident stem cells and are responsible for muscle regeneration. In this study we investigate the involvement of PKCε during muscle stem cell differentiation in vitro and in vivo. Here, we describe the identification of a previously unrecognized role for the PKCε–HMGA1 signaling axis in myoblast differentiation and regeneration processes.

Methods

PKCε expression was modulated in the C2C12 cell line and primary murine satellite cells in vitro, as well as in an in vivo model of muscle regeneration. Immunohistochemistry and immunofluorescence, RT-PCR and shRNA silencing techniques were used to determine the role of PKCε and HMGA1 in myogenic differentiation.

Results

PKCε expression increases and subsequently re-localizes to the nucleus during skeletal muscle cell differentiation. In the nucleus, PKCε blocks Hmga1 expression to promote Myogenin and Mrf4 accumulation and myoblast formation. Following in vivo muscle injury, PKCε accumulates in regenerating, centrally-nucleated myofibers. Pharmacological inhibition of PKCε impairs the expression of two crucial markers of muscle differentiation, namely MyoD and Myogenin, during injury induced muscle regeneration.

Conclusion

This work identifies the PKCε–HMGA1 signaling axis as a positive regulator of skeletal muscle differentiation.
Keyword PKCε
HMGA1
C2C12
Satellite cells
Skeletal muscle differentiation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biomedical Sciences Publications
 
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